Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PT723_RS20985 Genome accession   NZ_CP117971
Coordinates   4259198..4259638 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain B-1109     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4254198..4264638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT723_RS20965 aroP 4255196..4256569 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  PT723_RS20970 ampE 4256612..4257466 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  PT723_RS20975 ampD 4257463..4258014 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PT723_RS20980 nadC 4258102..4258995 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PT723_RS20985 pilA 4259198..4259638 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  PT723_RS20990 pilB 4259648..4261033 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  PT723_RS20995 hofC 4261023..4262225 (+) 1203 WP_000157266.1 protein transport protein HofC -
  PT723_RS21000 guaC 4262260..4263303 (-) 1044 WP_001217338.1 GMP reductase -
  PT723_RS21005 - 4263459..4263503 (-) 45 WP_120795372.1 protein YacM -
  PT723_RS21010 coaE 4263528..4264148 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=719951 PT723_RS20985 WP_000360904.1 4259198..4259638(+) (pilA) [Escherichia coli strain B-1109]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=719951 PT723_RS20985 WP_000360904.1 4259198..4259638(+) (pilA) [Escherichia coli strain B-1109]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363