Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MKL45_RS02495 Genome accession   NZ_CP102934
Coordinates   533649..534449 (+) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter sp. AOR07_HL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528649..539449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKL45_RS02470 (MKL45_02470) - 529559..530857 (+) 1299 WP_086374126.1 glycosyltransferase family 1 protein -
  MKL45_RS02475 (MKL45_02475) - 530882..531433 (+) 552 WP_005129748.1 phosphatase PAP2 family protein -
  MKL45_RS02480 (MKL45_02480) iscX 531533..531733 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  MKL45_RS02485 (MKL45_02485) ndk 531847..532278 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  MKL45_RS02490 (MKL45_02490) rlmN 532401..533633 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MKL45_RS02495 (MKL45_02495) pilF 533649..534449 (+) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MKL45_RS02500 (MKL45_02500) - 534440..535231 (+) 792 WP_005129742.1 RodZ domain-containing protein -
  MKL45_RS02505 (MKL45_02505) ispG 535253..536368 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MKL45_RS02510 (MKL45_02510) hisS 536365..537657 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  MKL45_RS02515 (MKL45_02515) - 537686..538384 (+) 699 WP_005129738.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=719946 MKL45_RS02495 WP_004738086.1 533649..534449(+) (pilF) [Acinetobacter sp. AOR07_HL]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=719946 MKL45_RS02495 WP_004738086.1 533649..534449(+) (pilF) [Acinetobacter sp. AOR07_HL]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726