Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NWE25_RS12325 Genome accession   NZ_CP102866
Coordinates   2586842..2587609 (-) Length   255 a.a.
NCBI ID   WP_038459238.1    Uniprot ID   -
Organism   Bacillus velezensis strain YS-AT-DS1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2581842..2592609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWE25_RS12305 (NWE25_12305) - 2582044..2582856 (-) 813 WP_038459234.1 pyruvate, water dikinase regulatory protein -
  NWE25_RS12310 (NWE25_12310) ccpN 2582882..2583520 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  NWE25_RS12315 (NWE25_12315) glyS 2583621..2585660 (-) 2040 WP_038459236.1 glycine--tRNA ligase subunit beta -
  NWE25_RS12320 (NWE25_12320) glyQ 2585653..2586540 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  NWE25_RS12325 (NWE25_12325) recO 2586842..2587609 (-) 768 WP_038459238.1 DNA repair protein RecO Machinery gene
  NWE25_RS12330 (NWE25_12330) - 2587646..2587789 (-) 144 WP_007408286.1 YqzL family protein -
  NWE25_RS12335 (NWE25_12335) era 2587945..2588850 (-) 906 WP_038459240.1 GTPase Era -
  NWE25_RS12340 (NWE25_12340) - 2588831..2589241 (-) 411 WP_003152976.1 cytidine deaminase -
  NWE25_RS12345 (NWE25_12345) - 2589365..2589739 (-) 375 WP_015240233.1 diacylglycerol kinase family protein -
  NWE25_RS12350 (NWE25_12350) ybeY 2589720..2590193 (-) 474 WP_038459242.1 rRNA maturation RNase YbeY -
  NWE25_RS12355 (NWE25_12355) - 2590194..2592326 (-) 2133 WP_007408284.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29262.93 Da        Isoelectric Point: 8.7972

>NTDB_id=719431 NWE25_RS12325 WP_038459238.1 2586842..2587609(-) (recO) [Bacillus velezensis strain YS-AT-DS1]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEVKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=719431 NWE25_RS12325 WP_038459238.1 2586842..2587609(-) (recO) [Bacillus velezensis strain YS-AT-DS1]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGTGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATACTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.627

100

0.886