Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PT257_RS02500 Genome accession   NZ_CP117818
Coordinates   542881..544275 (-) Length   464 a.a.
NCBI ID   WP_011213111.1    Uniprot ID   -
Organism   Legionella pneumophila strain D5916     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537881..549275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT257_RS02465 (PT257_02465) - 537951..538961 (-) 1011 WP_027226414.1 class I fructose-bisphosphate aldolase -
  PT257_RS02470 (PT257_02470) - 539111..539845 (-) 735 WP_011213109.1 ferredoxin--NADP reductase -
  PT257_RS02475 (PT257_02475) dotV 539929..540471 (+) 543 WP_010946221.1 type IV secretion system protein DotV -
  PT257_RS02480 (PT257_02480) - 540615..540899 (-) 285 WP_011945638.1 DUF3579 domain-containing protein -
  PT257_RS02485 (PT257_02485) pssA 541129..541866 (+) 738 WP_011213110.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  PT257_RS02490 (PT257_02490) - 542095..542364 (-) 270 WP_010946224.1 HPr family phosphocarrier protein -
  PT257_RS02495 (PT257_02495) hpf 542555..542854 (-) 300 WP_010946225.1 ribosome hibernation promoting factor -
  PT257_RS02500 (PT257_02500) rpoN/rpoN1 542881..544275 (-) 1395 WP_011213111.1 RNA polymerase factor sigma-54 Machinery gene
  PT257_RS02505 (PT257_02505) rpmG 544483..544647 (-) 165 WP_003635328.1 50S ribosomal protein L33 -
  PT257_RS02510 (PT257_02510) rpmB 544662..544898 (-) 237 WP_010946227.1 50S ribosomal protein L28 -
  PT257_RS02515 (PT257_02515) - 545128..546324 (-) 1197 WP_061722128.1 IS4-like element ISLpn1 family transposase -
  PT257_RS02520 (PT257_02520) trmB 546615..547328 (+) 714 WP_011213112.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  PT257_RS02525 (PT257_02525) - 547303..548427 (+) 1125 WP_041174029.1 M42 family metallopeptidase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52990.26 Da        Isoelectric Point: 5.3275

>NTDB_id=719359 PT257_RS02500 WP_011213111.1 542881..544275(-) (rpoN/rpoN1) [Legionella pneumophila strain D5916]
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNQDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVSEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDSGGE
CSSTAIRAVIKKLISTENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=719359 PT257_RS02500 WP_011213111.1 542881..544275(-) (rpoN/rpoN1) [Legionella pneumophila strain D5916]
ATGAAACCGACATTGCAGATTGGTATTGGTCAACATCTCACGTTAACCCCACAACTGCAACAAGCCATAAGACTGCTGCA
ATTATCCACCTTTGATTTACAACAAGAAATTCAACTCGTTGTTGAATCAAATCCTTTGCTTGAAGCCACTCCTGTTGAAG
AAAAAGAAGACGCCCAAATAAATACCAATCAGAACCAAGATGAATATTCAGATTTTCAATGGTCACAACTGTATAATAAC
TTAAACAAGCGCAATAATTTTGAAGAAAACGATTACAATTTTGATAATTTACACTGCACTACCACCAATTTACAAGATCA
TCTTAACTGGCAACTTGACCTGACGCCTATGAGCGATATTGACCGAGTCATTGCTACAACAATTATTGATGCGATAAACG
ATGACGGCTTTTTAACTATACCCGTTTCTGAAATTCACGCCAGCTTAAATAGCGATGATTTTCCTCTGGATATAGAAGAA
ATAGAAGCAGTGAGACACAGGCTTCAATTATTTGACCCCGTAGGATGCGCATCTGTTAATTTAGCAGAAACACTCGTTTT
GCAATTGGAGCAACTTCCACTGGAACCCAAGCTTTTGGCTACTGCCAAGAAAGTCATTCTTGAAGACATTGAGTTGCTAG
GGCAACATAACTACAGACAACTCATGAAAAATCATCAAATAAGTGAAAAAACAATTCATGAAATATTACAAGTCATTCAT
AAATTAAATCCAAAACCAGGAAGTTTGATCCATCAAGATAATATTGAATATGTTATTCCCGACTTAACTGTTAAAAAGAT
AGATAATCAATGGAAAGTTTTTTTGAATCAAGATATTCTTCCCAGGCTGAGTATCAATAGCCAATATGCCGCTTTAATCC
AACGCGCAAACAATAGTCTGGATAATCAATTCTTAAAGCATAATTTACAAGAAGCACGTTGGTTTTTAAAAAGCATTCAA
AGCCGTCAGGAAACACTTCTAAAAGTAGCAACCTGTATCATGGAATACCAACATGGATTTCTGGAATTTGGTGAAGAAGC
GATGAAACCATTAATATTGAATGATGTAGCTTCTGCCTTGGATATGCATGAATCAACGGTATCAAGAGTGACAACTCAAA
AATTTATTAACACTCCTCGAGGTGTTTTTGAGTTAAAGTACTTCTTCTCCAGCCATGTGAATACTGATAGCGGAGGAGAA
TGCTCCTCCACCGCAATCCGAGCGGTAATAAAAAAATTAATTTCAACAGAGAATAGAAAAAAACCTTTGAGTGATAGTAA
AATTGCCCAACTAATAGGTGAGCAAGGCATTAAAGTAGCAAGACGTACTGTTGCCAAATATCGCGAAGCAATGGGGATTC
CCCCATCCAATGAAAGAAAGGTTATTAGTAGTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(95-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-460)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

38.889

100

0.422