Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL263_RS00365 Genome accession   NZ_AP018938
Coordinates   60880..62241 (+) Length   453 a.a.
NCBI ID   WP_074017595.1    Uniprot ID   A0A237IXT5
Organism   Streptococcus pneumoniae strain ATCC 49619     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 55880..67241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL263_RS00315 (SPAT_0060) - 55965..56168 (+) 204 WP_001091109.1 hypothetical protein -
  EL263_RS00320 (SPAT_0061) - 56171..56521 (+) 351 WP_000852248.1 hypothetical protein -
  EL263_RS00325 (SPAT_0062) - 56531..56947 (+) 417 WP_001165341.1 phage holin family protein -
  EL263_RS00330 (SPAT_0063) - 56951..57283 (+) 333 WP_001186207.1 phage holin -
  EL263_RS00335 (SPAT_0064) lytA 57287..58243 (+) 957 WP_000350489.1 N-acetylmuramoyl-L-alanine amidase LytA -
  EL263_RS00340 (SPAT_0065) - 58512..58691 (-) 180 WP_001233269.1 hypothetical protein -
  EL263_RS11410 (SPAT_0066) - 58833..58982 (-) 150 WP_162489709.1 hypothetical protein -
  EL263_RS00345 (SPAT_0067) tadA 59252..59719 (+) 468 WP_000291874.1 tRNA adenosine(34) deaminase TadA -
  EL263_RS00355 (SPAT_0068) - 59906..60349 (+) 444 WP_000701992.1 dUTP diphosphatase -
  EL263_RS00360 (SPAT_0069) - 60351..60866 (+) 516 WP_000691236.1 histidine phosphatase family protein -
  EL263_RS00365 (SPAT_0070) radA 60880..62241 (+) 1362 WP_074017595.1 DNA repair protein RadA Machinery gene
  EL263_RS00370 (SPAT_0071) - 62314..62811 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  EL263_RS00380 (SPAT_0072) - 62836..63650 (+) 815 Protein_66 PrsW family intramembrane metalloprotease -
  EL263_RS00385 (SPAT_0073) - 63795..64763 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  EL263_RS11600 - 64881..65826 (+) 946 Protein_68 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.95 Da        Isoelectric Point: 6.5137

>NTDB_id=71922 EL263_RS00365 WP_074017595.1 60880..62241(+) (radA) [Streptococcus pneumoniae strain ATCC 49619]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=71922 EL263_RS00365 WP_074017595.1 60880..62241(+) (radA) [Streptococcus pneumoniae strain ATCC 49619]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAAGTTAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTTATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTAAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCGATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXC

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

100

100

1
  radA Streptococcus pneumoniae D39

100

100

1
  radA Streptococcus pneumoniae R6

100

100

1
  radA Streptococcus pneumoniae TIGR4

100

100

1
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment