Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JG732_RS01895 Genome accession   NZ_AP024205
Coordinates   416588..417265 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain TA8571     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411588..422265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG732_RS01880 (ECTA8571_03840) chiA 412955..415648 (+) 2694 WP_020239449.1 bifunctional chitinase/lysozyme -
  JG732_RS01885 (ECTA8571_03850) bfd 415817..416011 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JG732_RS01890 (ECTA8571_03860) bfr 416083..416559 (+) 477 WP_000675504.1 bacterioferritin -
  JG732_RS01895 (ECTA8571_03870) pilD 416588..417265 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JG732_RS01900 (ECTA8571_03880) gspM 417265..417726 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JG732_RS01905 (ECTA8571_03890) gspL 417723..418886 (-) 1164 WP_001618895.1 type II secretion system protein GspL -
  JG732_RS01910 (ECTA8571_03900) gspK 418901..419884 (-) 984 WP_063084446.1 type II secretion system minor pseudopilin GspK -
  JG732_RS01915 (ECTA8571_03910) gspJ 419877..420464 (-) 588 WP_000610633.1 type II secretion system minor pseudopilin GspJ -
  JG732_RS01920 (ECTA8571_03920) gspI 420457..420834 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JG732_RS01925 (ECTA8571_03930) gspH 420831..421340 (-) 510 WP_001618891.1 type II secretion system minor pseudopilin GspH -
  JG732_RS01930 (ECTA8571_03940) gspG 421348..421785 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=71896 JG732_RS01895 WP_000178161.1 416588..417265(-) (pilD) [Escherichia coli strain TA8571]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=71896 JG732_RS01895 WP_000178161.1 416588..417265(-) (pilD) [Escherichia coli strain TA8571]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment