Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   PTA75_RS00355 Genome accession   NZ_CP117720
Coordinates   73340..74008 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain B1W1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 68340..79008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTA75_RS00330 (PTA75_00330) pyrE 68865..69506 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  PTA75_RS00335 (PTA75_00335) - 69804..70763 (+) 960 WP_000340183.1 reverse transcriptase family protein -
  PTA75_RS00340 (PTA75_00340) slmA 70809..71405 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  PTA75_RS00345 (PTA75_00345) dut 71512..71970 (-) 459 WP_000976070.1 dUTP diphosphatase -
  PTA75_RS00350 (PTA75_00350) coaBC 71948..73168 (-) 1221 WP_000050158.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  PTA75_RS00355 (PTA75_00355) radC 73340..74008 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  PTA75_RS00360 (PTA75_00360) rpmB 74225..74461 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  PTA75_RS00365 (PTA75_00365) rpmG 74482..74649 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  PTA75_RS00370 (PTA75_00370) mutM 74747..75556 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  PTA75_RS00375 (PTA75_00375) coaD 75595..76074 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  PTA75_RS00380 (PTA75_00380) waaA 76082..77359 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  PTA75_RS00385 (PTA75_00385) rfaQ 77772..78830 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=718741 PTA75_RS00355 WP_001297375.1 73340..74008(+) (radC) [Escherichia coli strain B1W1]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=718741 PTA75_RS00355 WP_001297375.1 73340..74008(+) (radC) [Escherichia coli strain B1W1]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGTTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496