Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL271_RS00145 Genome accession   NZ_AP018937
Coordinates   25171..26532 (+) Length   453 a.a.
NCBI ID   WP_074017595.1    Uniprot ID   A0A237IXT5
Organism   Streptococcus pneumoniae strain HU-OH     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20171..31532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL271_RS00105 - 20283..20492 (+) 210 Protein_14 transposase -
  EL271_RS00110 - 20527..21324 (-) 798 Protein_15 transposase -
  EL271_RS00115 (SPOH_0019) comW 21590..21826 (+) 237 WP_000939546.1 sigma(X)-activator ComW Regulator
  EL271_RS00120 (SPOH_0020) - 22057..23343 (+) 1287 WP_000205044.1 adenylosuccinate synthase -
  EL271_RS00125 (SPOH_0021) tadA 23544..24011 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  EL271_RS00135 (SPOH_0022) - 24197..24640 (+) 444 WP_000701992.1 dUTP diphosphatase -
  EL271_RS00140 (SPOH_0023) - 24642..25157 (+) 516 WP_001838385.1 histidine phosphatase family protein -
  EL271_RS00145 (SPOH_0024) radA 25171..26532 (+) 1362 WP_074017595.1 DNA repair protein RadA Machinery gene
  EL271_RS00150 (SPOH_0025) - 26605..27102 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  EL271_RS00160 (SPOH_0027) - 27127..27957 (+) 831 Protein_23 PrsW family glutamic-type intramembrane protease -
  EL271_RS00165 (SPOH_0028) - 28102..29070 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  EL271_RS00170 - 29207..29485 (-) 279 Protein_25 transposase family protein -
  EL271_RS11560 - 29529..30454 (-) 926 Protein_26 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.95 Da        Isoelectric Point: 6.5137

>NTDB_id=71848 EL271_RS00145 WP_074017595.1 25171..26532(+) (radA) [Streptococcus pneumoniae strain HU-OH]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=71848 EL271_RS00145 WP_074017595.1 25171..26532(+) (radA) [Streptococcus pneumoniae strain HU-OH]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTTGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXC

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

100

100

1
  radA Streptococcus pneumoniae D39

100

100

1
  radA Streptococcus pneumoniae R6

100

100

1
  radA Streptococcus pneumoniae TIGR4

100

100

1
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment