Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NUH86_RS00790 Genome accession   NZ_CP102664
Coordinates   158136..159503 (+) Length   455 a.a.
NCBI ID   WP_267250807.1    Uniprot ID   -
Organism   Sphingobium sp. JS3065     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 153136..164503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUH86_RS00765 (NUH86_00765) - 153556..153981 (-) 426 WP_267250802.1 metallopeptidase family protein -
  NUH86_RS00770 (NUH86_00770) - 154066..154353 (+) 288 WP_267250803.1 4a-hydroxytetrahydrobiopterin dehydratase -
  NUH86_RS00775 (NUH86_00775) - 154345..154953 (-) 609 WP_267250804.1 hypothetical protein -
  NUH86_RS00780 (NUH86_00780) - 154950..157283 (-) 2334 WP_267250805.1 patatin-like protein -
  NUH86_RS00785 (NUH86_00785) - 157348..158046 (-) 699 WP_267250806.1 endonuclease/exonuclease/phosphatase family protein -
  NUH86_RS00790 (NUH86_00790) radA 158136..159503 (+) 1368 WP_267250807.1 DNA repair protein RadA Machinery gene
  NUH86_RS00795 (NUH86_00795) - 159515..160036 (+) 522 WP_267250808.1 CvpA family protein -
  NUH86_RS00800 (NUH86_00800) - 160046..160195 (-) 150 WP_267252191.1 zinc-binding dehydrogenase -
  NUH86_RS00805 (NUH86_00805) - 160180..160425 (-) 246 WP_267252204.1 hypothetical protein -
  NUH86_RS00810 (NUH86_00810) - 160510..161034 (-) 525 WP_267250809.1 enoyl-CoA hydratase-related protein -
  NUH86_RS00815 (NUH86_00815) - 161130..161906 (-) 777 WP_267252192.1 SDR family oxidoreductase -
  NUH86_RS00820 (NUH86_00820) - 162100..162516 (-) 417 WP_267250810.1 BLUF domain-containing protein -
  NUH86_RS00825 (NUH86_00825) - 162744..163358 (+) 615 WP_267250811.1 PilZ domain-containing protein -
  NUH86_RS00830 (NUH86_00830) - 163442..164053 (+) 612 WP_267250812.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 47965.89 Da        Isoelectric Point: 6.7633

>NTDB_id=718311 NUH86_RS00790 WP_267250807.1 158136..159503(+) (radA) [Sphingobium sp. JS3065]
MAKAKRKFVCQQCGTVSSRWQGQCDDCGEWNSIVEEAAETVFSARHDLHSGGRALRLVGLDDKVELPPRTTTGIAEFDRA
LGGGIVSGSATLIGGDPGIGKSTLLLQAAARIATAGHRVAYISGEEAADQVRLRAQRLGLGNAPVQLASATSVRDILTTL
GEEAPPALLIIDSIQTMHSDLIEGAPGTVSQVRASSQELIRFAKQRGTALILVGHVTKDGSIAGPRVLEHMVDTVLSFEG
ERSHQYRILRAIKNRFGGTDEIGVFAMVAEGLEEVANPSALFLTHRDENVTGATVFPALEGTRPVLVEIQALVVRLSSGA
TPRRAVVGWDSGRLAMVLAVLEARCGLSFSTCEVYLNVAGGYRLSDPAADLAVAAALMSALSERPVPADVVLFGEIALSG
EIRPVAHAPLRLRESAKLGFNRAFVPAAVADGVKGISVSGYRALAQLVDQMLGRG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=718311 NUH86_RS00790 WP_267250807.1 158136..159503(+) (radA) [Sphingobium sp. JS3065]
ATGGCAAAGGCAAAGCGCAAATTCGTCTGTCAGCAATGCGGCACCGTTTCCAGCCGATGGCAGGGACAATGCGACGATTG
CGGCGAATGGAACAGCATCGTCGAGGAAGCGGCGGAGACGGTCTTTTCCGCCCGCCACGACCTGCATAGCGGGGGGCGCG
CGCTCAGGCTGGTCGGCCTGGACGACAAGGTCGAACTGCCGCCGCGCACCACCACCGGCATCGCGGAATTCGACCGGGCG
CTGGGCGGCGGCATCGTGTCCGGCTCCGCCACGCTGATCGGCGGCGACCCCGGCATCGGCAAATCGACGCTGCTGCTCCA
GGCCGCCGCCCGCATCGCCACAGCGGGACACCGCGTCGCATACATCAGCGGCGAGGAAGCGGCCGATCAGGTCCGCCTGC
GCGCCCAGCGGCTGGGCCTGGGCAATGCGCCGGTGCAACTCGCCAGTGCCACCTCCGTCCGCGACATATTGACGACGCTG
GGGGAGGAGGCGCCGCCCGCCCTGCTCATCATCGATTCGATCCAGACCATGCACAGCGACCTGATCGAGGGCGCGCCCGG
CACGGTCAGCCAGGTGCGCGCCTCCAGCCAGGAACTGATCCGCTTCGCCAAGCAGCGCGGCACGGCGCTGATCCTGGTCG
GCCATGTGACCAAGGACGGCAGCATCGCCGGCCCCCGCGTGCTGGAACATATGGTCGACACGGTGCTGAGCTTCGAGGGC
GAGCGCAGCCATCAATATCGCATCCTGCGCGCCATCAAGAACCGCTTCGGCGGCACGGATGAGATTGGCGTCTTCGCCAT
GGTCGCGGAGGGGCTGGAGGAAGTCGCCAATCCCTCCGCCCTGTTTCTCACCCATCGCGACGAGAATGTGACCGGCGCGA
CGGTCTTCCCCGCGCTGGAAGGCACGCGCCCGGTGCTGGTCGAGATCCAGGCGCTGGTCGTCCGCCTTTCCAGCGGCGCC
ACCCCTCGCCGTGCGGTGGTCGGGTGGGACAGCGGACGGCTTGCCATGGTGCTTGCCGTGCTGGAGGCGCGATGCGGGCT
CAGCTTCTCGACCTGCGAAGTCTATCTCAATGTCGCGGGCGGCTATCGCCTGTCGGACCCCGCCGCCGACCTGGCCGTCG
CCGCCGCGCTCATGTCCGCCTTGTCGGAACGTCCGGTTCCCGCCGATGTCGTCCTCTTCGGCGAAATCGCGTTGTCCGGC
GAAATTCGTCCTGTCGCCCATGCCCCACTCCGGCTGCGCGAATCCGCCAAGCTGGGCTTCAACCGCGCCTTCGTGCCCGC
CGCCGTGGCCGATGGGGTGAAGGGCATTTCGGTCAGCGGCTATCGCGCCCTTGCGCAACTTGTTGACCAGATGCTCGGGC
GCGGATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(82-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

47.619

100

0.484
  radA Streptococcus mitis NCTC 12261

46.171

100

0.464
  radA Streptococcus pneumoniae Rx1

45.952

100

0.462
  radA Streptococcus pneumoniae D39

45.952

100

0.462
  radA Streptococcus pneumoniae R6

45.952

100

0.462
  radA Streptococcus pneumoniae TIGR4

45.952

100

0.462
  radA Streptococcus mitis SK321

47.356

95.604

0.453