Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PSR65_RS06435 Genome accession   NZ_CP117469
Coordinates   1342173..1343606 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain B-ZidR     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1337173..1348606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSR65_RS06400 (PSR65_06390) elbB 1337864..1338517 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  PSR65_RS06405 (PSR65_06395) mtgA 1338514..1339242 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PSR65_RS06410 (PSR65_06400) yrbL 1339239..1339871 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  PSR65_RS06415 (PSR65_06405) npr 1340085..1340357 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PSR65_RS06420 (PSR65_06410) rapZ 1340354..1341208 (-) 855 WP_000243741.1 RNase adapter RapZ -
  PSR65_RS06425 (PSR65_06415) ptsN 1341254..1341745 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PSR65_RS06430 (PSR65_06420) hpf 1341863..1342150 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PSR65_RS06435 (PSR65_06425) rpoN/rpoN1 1342173..1343606 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PSR65_RS06440 (PSR65_06430) lptB 1343654..1344379 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PSR65_RS06445 (PSR65_06435) lptA 1344386..1344943 (-) 558 WP_000669774.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PSR65_RS06450 (PSR65_06440) lptC 1344912..1345487 (-) 576 WP_000030530.1 LPS export ABC transporter periplasmic protein LptC -
  PSR65_RS06455 (PSR65_06445) kdsC 1345484..1346050 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PSR65_RS06460 (PSR65_06450) kdsD 1346071..1347057 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PSR65_RS06465 (PSR65_06455) yrbG 1347071..1348048 (-) 978 WP_000922900.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=718196 PSR65_RS06435 WP_000809051.1 1342173..1343606(-) (rpoN/rpoN1) [Escherichia coli strain B-ZidR]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=718196 PSR65_RS06435 WP_000809051.1 1342173..1343606(-) (rpoN/rpoN1) [Escherichia coli strain B-ZidR]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465