Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PSH70_RS04495 Genome accession   NZ_CP117438
Coordinates   1041549..1043042 (-) Length   497 a.a.
NCBI ID   WP_053254420.1    Uniprot ID   A0ABY1TCC4
Organism   Pseudomonas fluorescens strain FP2327     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1036549..1048042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH70_RS04460 (PSH70_04460) - 1037538..1037966 (+) 429 WP_053254415.1 hypothetical protein -
  PSH70_RS04465 (PSH70_04465) - 1037968..1038579 (+) 612 WP_053254416.1 superoxide dismutase -
  PSH70_RS04470 (PSH70_04470) - 1038611..1039504 (+) 894 WP_053254417.1 ZIP family metal transporter -
  PSH70_RS04475 (PSH70_04475) - 1039542..1039814 (-) 273 WP_049709983.1 HPr family phosphocarrier protein -
  PSH70_RS04480 (PSH70_04480) rapZ 1039831..1040688 (-) 858 WP_053254418.1 RNase adapter RapZ -
  PSH70_RS04485 (PSH70_04485) ptsN 1040691..1041155 (-) 465 WP_201229864.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PSH70_RS04490 (PSH70_04490) raiA 1041168..1041473 (-) 306 WP_017138237.1 ribosome-associated translation inhibitor RaiA -
  PSH70_RS04495 (PSH70_04495) rpoN/rpoN1 1041549..1043042 (-) 1494 WP_053254420.1 RNA polymerase factor sigma-54 Machinery gene
  PSH70_RS04500 (PSH70_04500) lptB 1043227..1043952 (-) 726 WP_053254421.1 LPS export ABC transporter ATP-binding protein -
  PSH70_RS04505 (PSH70_04505) lptA 1043952..1044527 (-) 576 WP_053254422.1 lipopolysaccharide transport periplasmic protein LptA -
  PSH70_RS04510 (PSH70_04510) lptC 1044514..1045086 (-) 573 WP_069552864.1 LPS export ABC transporter periplasmic protein LptC -
  PSH70_RS04515 (PSH70_04515) - 1045095..1045619 (-) 525 WP_069552865.1 HAD family hydrolase -
  PSH70_RS04520 (PSH70_04520) - 1045620..1046594 (-) 975 WP_053254425.1 KpsF/GutQ family sugar-phosphate isomerase -
  PSH70_RS04525 (PSH70_04525) - 1046894..1047703 (+) 810 WP_053254426.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56640.78 Da        Isoelectric Point: 4.3925

>NTDB_id=718089 PSH70_RS04495 WP_053254420.1 1041549..1043042(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain FP2327]
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEEGDDFDNADPLADNIEQKPNSDVQEPSYQ
ETAPTVDNLEEGEWNERIPNELPVDTAWEDVYQTSASSLPSNDDDEWDFTTRTSVGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLEEILEAFDPELDIELDEIEAVLHRIQQFEPAGIGARTLSECLLLQLRQLPAKTLWLAEAQRLV
TDYIDLLGSRDYSQLMRRMKLKEDDLRQVIELVQSLNPRPGSQIESSEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
PQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=718089 PSH70_RS04495 WP_053254420.1 1041549..1043042(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain FP2327]
ATGAAACCATCGCTAGTCCTGAGAATGGGCCAGCAGCTGACGATGACACCGCAGCTGCAACAGGCCATCCGCCTGCTCCA
ATTGTCGACCCTGGACCTGCAACAGGAAATCCAGGAGGCCCTGGAGTCCAATCCGATGCTCGAACGCCAGGAAGAAGGCG
ACGACTTCGATAATGCAGACCCGCTGGCCGACAACATCGAGCAAAAACCCAACTCCGACGTGCAGGAACCTTCCTATCAG
GAAACCGCCCCTACGGTGGACAACCTTGAGGAAGGCGAATGGAACGAACGCATTCCCAACGAGCTTCCGGTGGACACCGC
CTGGGAAGACGTTTACCAGACCAGCGCCAGCAGCCTGCCCAGCAATGATGATGACGAGTGGGACTTCACCACCCGCACCT
CCGTCGGCGAAAGCCTGCAGAGCCATTTGCTGTGGCAATTGAACCTGGCGCCGATGTCCGACACCGATCGCCTGATCGCT
GTGACCCTGATCGACTGCATCAATAACCAGGGTTACCTCGACGAGACGCTCGAAGAGATCCTTGAGGCTTTCGATCCGGA
ACTGGACATCGAACTGGACGAAATCGAAGCCGTCCTGCACCGCATCCAGCAGTTCGAGCCCGCCGGCATCGGCGCCCGCA
CACTGAGCGAGTGCCTGCTGCTGCAACTGCGCCAGCTGCCCGCCAAGACCCTGTGGCTCGCCGAAGCCCAGCGCCTGGTC
ACCGACTACATCGACCTGCTGGGCAGCCGCGACTACAGCCAGTTGATGCGCCGCATGAAGCTCAAGGAAGACGACCTGCG
CCAGGTCATCGAGCTGGTACAGAGCCTTAACCCCCGCCCGGGCTCGCAGATCGAGTCCAGCGAAGCCGAATACGTCGTTC
CCGATGTAATCGTGCGCAAAGACAACGAGCGTTGGCTGGTGGAACTGAACCAGGAGTCGGTGCCTCGCCTGCGCGTCAAC
CCGCAATACGCAGGTTTTGTGCGCCGTGCCGACACCAGTGCCGATAACACCTTCATGCGCAATCAGTTGCAGGAAGCGCG
CTGGTTCATCAAGAGCCTGCAAAGCCGCAACGAAACCCTGATGAAAGTGGCCACTCAGATCGTCGAGCACCAGCGCGGCT
TCCTCGAATATGGTGACGAGGCCATGAAACCTTTGGTCCTGCACGATATCGCTGAGGCGGTTGGCATGCATGAATCGACG
ATTTCCCGGGTAACCACGCAGAAATTCATGCATACCCCACGGGGTATTTATGAGCTGAAATACTTTTTCTCCAGCCACGT
AAGTACCTCCGAAGGCGGCGAATGCTCGTCCACGGCGATCCGCGCGATCATTAAAAAACTGGTTGCCGCGGAAAATCAGA
AAAAGCCGTTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAAGGCATTCAGGTCGCCCGTCGAACCGTCGCCAAG
TACCGCGAGTCCCTCGGGATCGCGCCTTCCAGCGAGCGTAAGCGTTTGATGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.554

100

0.453