Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NUW85_RS09855 Genome accession   NZ_CP102603
Coordinates   2094398..2094835 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MBLB0692     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2089398..2099835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUW85_RS09840 (NUW85_09845) - 2092256..2092618 (-) 363 WP_014304320.1 helix-turn-helix domain-containing protein -
  NUW85_RS09845 (NUW85_09850) hxlA 2092850..2093485 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  NUW85_RS09850 (NUW85_09855) hxlB 2093482..2094039 (+) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  NUW85_RS09855 (NUW85_09860) nucA/comI 2094398..2094835 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NUW85_RS09860 (NUW85_09865) nin/comJ 2094857..2095255 (+) 399 WP_003156589.1 competence protein ComJ Regulator
  NUW85_RS09865 (NUW85_09870) - 2095296..2095514 (-) 219 Protein_1936 family 1 glycosylhydrolase -
  NUW85_RS09870 (NUW85_09875) - 2095733..2096053 (-) 321 WP_139885554.1 YckD family protein -
  NUW85_RS09875 (NUW85_09880) - 2096125..2096559 (-) 435 WP_021494203.1 RDD family protein -
  NUW85_RS09880 (NUW85_09885) - 2096574..2097710 (-) 1137 WP_088037494.1 zinc-dependent alcohol dehydrogenase -
  NUW85_RS09885 (NUW85_09890) - 2097775..2098977 (-) 1203 WP_015388720.1 GTP-binding protein -
  NUW85_RS09890 (NUW85_09895) - 2099130..2099255 (+) 126 Protein_1941 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=718044 NUW85_RS09855 WP_003156588.1 2094398..2094835(+) (nucA/comI) [Bacillus amyloliquefaciens strain MBLB0692]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=718044 NUW85_RS09855 WP_003156588.1 2094398..2094835(+) (nucA/comI) [Bacillus amyloliquefaciens strain MBLB0692]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641