Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NTJ56_RS15935 Genome accession   NZ_CP102482
Coordinates   3346484..3347989 (+) Length   501 a.a.
NCBI ID   WP_226117206.1    Uniprot ID   -
Organism   Burkholderia contaminans strain DM32     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3341484..3352989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTJ56_RS15910 (NTJ56_15910) kdsD 3342728..3343711 (+) 984 WP_039345999.1 arabinose 5-phosphate isomerase KdsD -
  NTJ56_RS15915 (NTJ56_15915) - 3343711..3344247 (+) 537 WP_071335102.1 HAD family hydrolase -
  NTJ56_RS15920 (NTJ56_15920) lptC 3344251..3344865 (+) 615 WP_226117210.1 LPS export ABC transporter periplasmic protein LptC -
  NTJ56_RS15925 (NTJ56_15925) lptA 3344886..3345551 (+) 666 WP_226221305.1 lipopolysaccharide transport periplasmic protein LptA -
  NTJ56_RS15930 (NTJ56_15930) lptB 3345548..3346324 (+) 777 WP_039346017.1 LPS export ABC transporter ATP-binding protein -
  NTJ56_RS15935 (NTJ56_15935) rpoN/rpoN1 3346484..3347989 (+) 1506 WP_226117206.1 RNA polymerase factor sigma-54 Machinery gene
  NTJ56_RS15940 (NTJ56_15940) hpf 3348148..3348507 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NTJ56_RS15945 (NTJ56_15945) - 3348676..3349215 (+) 540 WP_011353226.1 PTS sugar transporter subunit IIA -
  NTJ56_RS15950 (NTJ56_15950) hprK 3349345..3350313 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  NTJ56_RS15955 (NTJ56_15955) rapZ 3350389..3351297 (+) 909 WP_226117204.1 RNase adapter RapZ -
  NTJ56_RS15960 (NTJ56_15960) - 3351325..3351960 (+) 636 WP_039346030.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54771.86 Da        Isoelectric Point: 4.8740

>NTDB_id=717239 NTJ56_RS15935 WP_226117206.1 3346484..3347989(+) (rpoN/rpoN1) [Burkholderia contaminans strain DM32]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHSLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=717239 NTJ56_RS15935 WP_226117206.1 3346484..3347989(+) (rpoN/rpoN1) [Burkholderia contaminans strain DM32]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCTGCGGACGGATCGCTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAGCCCACTGCG
ACAAACGGTAGCAGTTCGTCGAGCGATCGTGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACTCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCTTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTTTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691