Detailed information NTG0717198

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: NUJ27_RS16080
• Genome accession: NZ_CP102475
• Coordinates: 3377715..3378875 (+)
• Length: 386 a.a.
• NCBI ID: WP_006483736.1
• Uniprot ID: B4EAQ6
• Organism: Burkholderia cenocepacia strain NML110041
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3372715..3383875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NUJ27_RS16050 (NUJ27_16050)	-	3373362..3373967 (+)	606	WP_006483734.1	50S ribosomal protein L25/general stress protein Ctc	-
NUJ27_RS16055 (NUJ27_16055)	pth	3374088..3374687 (+)	600	WP_006477776.1	aminoacyl-tRNA hydrolase	-
NUJ27_RS16060 (NUJ27_16060)	hisC	3374804..3375871 (+)	1068	WP_006483731.1	histidinol-phosphate transaminase	-
NUJ27_RS16065 (NUJ27_16065)	-	3375949..3376215 (-)	267	WP_006483745.1	YfhL family 4Fe-4S dicluster ferredoxin	-
NUJ27_RS16070 (NUJ27_16070)	coaD	3376275..3376772 (-)	498	WP_006477773.1	pantetheine-phosphate adenylyltransferase	-
NUJ27_RS16075 (NUJ27_16075)	rsmD	3376884..3377498 (-)	615	WP_006483748.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
NUJ27_RS16080 (NUJ27_16080)	pilA	3377715..3378875 (+)	1161	WP_006483736.1	signal recognition particle-docking protein FtsY	Machinery gene
NUJ27_RS16085 (NUJ27_16085)	maiA	3378961..3379605 (-)	645	WP_006499022.1	maleylacetoacetate isomerase	-
NUJ27_RS16090 (NUJ27_16090)	ybiB	3379785..3380759 (+)	975	WP_006483732.1	DNA-binding protein YbiB	-
NUJ27_RS16095 (NUJ27_16095)	leuA	3381128..3382774 (+)	1647	WP_006483729.1	2-isopropylmalate synthase	-
NUJ27_RS16100 (NUJ27_16100)	-	3382918..3383301 (-)	384	WP_006483747.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 40953.90 Da
• Isoelectric Point: 4.7940

>NTDB_id=717198 NUJ27_RS16080 WP_006483736.1 3377715..3378875(+) (pilA) [Burkholderia cenocepacia strain NML110041]
MFSFFKRFKKTQEPDPAESQSADAQQADEASDADAPVLDAPPAASVPPAPAQPAAPAVVMTVTPTNDGRDEVVETVEIVP
PPLQDASAKKSWLARLKTGLAKTGSSITGVFVNTKIDEDLYEELETALLMSDAGVDATEYLLGALREKVRAGRLTDPQQV
KAALHDLLVELLTPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVIAKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAVEFADALLG

Nucleotide

• Length: 1161 bp

>NTDB_id=717198 NUJ27_RS16080 WP_006483736.1 3377715..3378875(+) (pilA) [Burkholderia cenocepacia strain NML110041]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGATCCGGCGGAATCGCAATCGGCCGACGCGCAGCAAGC
GGACGAAGCGTCCGACGCCGACGCGCCCGTGCTCGACGCGCCGCCCGCGGCCAGCGTGCCGCCAGCGCCCGCGCAACCGG
CTGCGCCGGCCGTCGTGATGACCGTCACGCCGACCAACGACGGCCGCGACGAAGTCGTCGAGACGGTCGAGATCGTGCCG
CCGCCGCTGCAGGACGCGTCCGCGAAGAAGTCGTGGCTCGCGCGCCTGAAGACGGGGCTCGCGAAGACGGGCTCGAGCAT
CACCGGCGTCTTCGTCAACACGAAGATCGACGAGGATCTGTACGAGGAGCTCGAAACCGCGCTGCTGATGTCCGACGCGG
GCGTCGACGCGACCGAATACCTGCTCGGCGCGCTGCGCGAAAAGGTGCGCGCGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGGCTGCGCTGCACGACCTGCTCGTCGAGCTGCTGACGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACCGGCGTGAACGGCGCAGGCAAGACGACCAGCATCGGCAAGCTCGCGAAGCACCTGCAGAGCTTCG
ACCAGTCGGTGCTGCTGGCTGCCGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTGCAGCAGGAGAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCATCTGATGGAGGAGCTGAAGAAGGTGAAGC
GCGTGATCGCGAAGGCGCACGACGGCGCGCCGCACGAAGTGCTGCTGGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGACGCGCTGGGCCTGACCGGCCTGATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGG
GATTCTCGCCGCGATCGCGCGGCAACGGCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAGCGCGGTGGAATTCGCGGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-386)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

3D structure

• AlphaFold DB: B4EAQ6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.606	100	0.578