Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NUJ27_RS15985 Genome accession   NZ_CP102475
Coordinates   3360911..3362416 (+) Length   501 a.a.
NCBI ID   WP_006483132.1    Uniprot ID   -
Organism   Burkholderia cenocepacia strain NML110041     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3355911..3367416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUJ27_RS15960 (NUJ27_15960) kdsD 3357142..3358125 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  NUJ27_RS15965 (NUJ27_15965) - 3358125..3358661 (+) 537 WP_006483148.1 HAD family hydrolase -
  NUJ27_RS15970 (NUJ27_15970) lptC 3358665..3359279 (+) 615 WP_006483145.1 LPS export ABC transporter periplasmic protein LptC -
  NUJ27_RS15975 (NUJ27_15975) lptA 3359300..3359965 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  NUJ27_RS15980 (NUJ27_15980) lptB 3359962..3360738 (+) 777 WP_006483143.1 LPS export ABC transporter ATP-binding protein -
  NUJ27_RS15985 (NUJ27_15985) rpoN/rpoN1 3360911..3362416 (+) 1506 WP_006483132.1 RNA polymerase factor sigma-54 Machinery gene
  NUJ27_RS15990 (NUJ27_15990) hpf 3362575..3362934 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NUJ27_RS15995 (NUJ27_15995) - 3363130..3363642 (+) 513 Protein_3127 PTS sugar transporter subunit IIA -
  NUJ27_RS16000 (NUJ27_16000) hprK 3363772..3364740 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  NUJ27_RS16005 (NUJ27_16005) rapZ 3364815..3365723 (+) 909 WP_006483144.1 RNase adapter RapZ -
  NUJ27_RS16010 (NUJ27_16010) - 3365750..3366385 (+) 636 WP_006483137.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54735.87 Da        Isoelectric Point: 4.8740

>NTDB_id=717197 NUJ27_RS15985 WP_006483132.1 3360911..3362416(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain NML110041]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNGSSPSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLAAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=717197 NUJ27_RS15985 WP_006483132.1 3360911..3362416(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain NML110041]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCGCCGCG
ACCAACGGCAGCAGCCCGTCCAGCGATCGCGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTCGCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACTGCGACGAACTCAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGTGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTTGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCACTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAAGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689