Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NUJ28_RS14530 Genome accession   NZ_CP102458
Coordinates   3007975..3009480 (+) Length   501 a.a.
NCBI ID   WP_176034321.1    Uniprot ID   -
Organism   Burkholderia multivorans strain NML180963     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3002975..3014480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUJ28_RS14505 (NUJ28_14505) kdsD 3004226..3005209 (+) 984 WP_006398260.1 arabinose 5-phosphate isomerase KdsD -
  NUJ28_RS14510 (NUJ28_14510) - 3005209..3005745 (+) 537 WP_006398259.1 HAD family hydrolase -
  NUJ28_RS14515 (NUJ28_14515) lptC 3005749..3006363 (+) 615 WP_006398258.1 LPS export ABC transporter periplasmic protein LptC -
  NUJ28_RS14520 (NUJ28_14520) lptA 3006384..3007049 (+) 666 WP_176034322.1 lipopolysaccharide transport periplasmic protein LptA -
  NUJ28_RS14525 (NUJ28_14525) lptB 3007046..3007822 (+) 777 WP_006398256.1 LPS export ABC transporter ATP-binding protein -
  NUJ28_RS14530 (NUJ28_14530) rpoN/rpoN1 3007975..3009480 (+) 1506 WP_176034321.1 RNA polymerase factor sigma-54 Machinery gene
  NUJ28_RS14535 (NUJ28_14535) hpf 3009639..3009998 (+) 360 WP_006398254.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NUJ28_RS14540 (NUJ28_14540) - 3010193..3010705 (+) 513 WP_035488861.1 PTS sugar transporter subunit IIA -
  NUJ28_RS14545 (NUJ28_14545) hprK 3010834..3011802 (+) 969 WP_006398252.1 HPr(Ser) kinase/phosphatase -
  NUJ28_RS14550 (NUJ28_14550) rapZ 3011881..3012789 (+) 909 WP_006409809.1 RNase adapter RapZ -
  NUJ28_RS14555 (NUJ28_14555) - 3012817..3013452 (+) 636 WP_006398250.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54776.96 Da        Isoelectric Point: 4.8740

>NTDB_id=717152 NUJ28_RS14530 WP_176034321.1 3007975..3009480(+) (rpoN/rpoN1) [Burkholderia multivorans strain NML180963]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVSNGEPAI
ANGNGQSSDRAERDEPTGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=717152 NUJ28_RS14530 WP_176034321.1 3007975..3009480(+) (rpoN/rpoN1) [Burkholderia multivorans strain NML180963]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGTCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGTGCGGAGCGCGACGAACCGACCGGCGCCGACGAATACGACGGCTATGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGACGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCGAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGACGAACTGAACGCGGCGCTCGCACTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGTTGCTGCGGCTGGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCGCTGCGCGACGCGCACGCGCTGATTCGCTCGCTCGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAAGTCGGCCAGGGCTGGCAGGCCGAGCTCAATCCCGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GTTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695