Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   G314FT_RS03755 Genome accession   NZ_CP102451
Coordinates   716588..717958 (+) Length   456 a.a.
NCBI ID   WP_257702116.1    Uniprot ID   -
Organism   Vagococcus luciliae strain G314F     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 711588..722958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G314FT_RS03740 (G314FT_07500) proB 712831..713634 (-) 804 WP_257702114.1 glutamate 5-kinase -
  G314FT_RS03745 (G314FT_07510) - 713880..715862 (+) 1983 WP_257702497.1 KUP/HAK/KT family potassium transporter -
  G314FT_RS03750 (G314FT_07520) - 715951..716478 (+) 528 WP_257702115.1 dUTP diphosphatase -
  G314FT_RS03755 (G314FT_07530) radA 716588..717958 (+) 1371 WP_257702116.1 DNA repair protein RadA Machinery gene
  G314FT_RS03760 (G314FT_07540) - 717979..719064 (+) 1086 WP_257702117.1 PIN/TRAM domain-containing protein -
  G314FT_RS03765 (G314FT_07550) gltX 719165..720640 (+) 1476 WP_257702118.1 glutamate--tRNA ligase -
  G314FT_RS03770 (G314FT_07560) cysS 720888..722306 (+) 1419 WP_257702119.1 cysteine--tRNA ligase -
  G314FT_RS03775 (G314FT_07570) - 722303..722722 (+) 420 WP_257702120.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50057.51 Da        Isoelectric Point: 7.3272

>NTDB_id=717060 G314FT_RS03755 WP_257702116.1 716588..717958(+) (radA) [Vagococcus luciliae strain G314F]
MAKKKRNEFVCQTCGYISPKYLGRCPNCGEWNTLVEEIPSDTPTRHSRVSLTGEMSKPQKLKEVATQKERRIKTQLGELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLSLLKGNVLYVSGEESSQQIKMRAERLADGTNDFYVYPETDMGRIKQ
VIEQLTPDYVIIDSIQTMVHPETESAAGSVSQVRANTAELMQIAKTNNIAIFIVGHVTKEGSLAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMRQAGLVEVLNPSEAFLEERIDGATGSAIVCSMEGTRPILAEIQSLVTPSVFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAVGGVKLDEPAIDLSIAVSIASSYKDKGTSPQDCFIGEIGLTG
EIRRVNRIEQRVGEAQKLGFRRVFIPINNLQGWTPPTGIEVIGVETLSETLFKVFS

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=717060 G314FT_RS03755 WP_257702116.1 716588..717958(+) (radA) [Vagococcus luciliae strain G314F]
GTGGCAAAAAAAAAGAGAAATGAGTTTGTTTGTCAAACGTGTGGTTATATCTCTCCAAAGTATCTGGGTCGTTGTCCTAA
CTGTGGTGAATGGAATACATTAGTGGAAGAAATTCCTTCAGATACCCCCACTAGACATAGTCGAGTAAGTTTGACGGGTG
AAATGAGCAAACCTCAAAAGTTAAAAGAAGTCGCTACACAAAAAGAACGGCGTATAAAAACACAATTAGGTGAATTAAAT
AGAGTATTAGGCGGGGGTGTTGTTCCAGGATCAATGGTCTTAATTGGTGGAGATCCTGGTATTGGAAAATCAACACTATT
ACTACAAGTATCTCAACAACTTAGCCTGTTAAAAGGAAATGTGTTATATGTATCTGGTGAAGAGAGTTCGCAACAGATTA
AAATGCGTGCAGAACGCTTGGCTGATGGGACAAATGATTTTTATGTTTATCCAGAAACGGATATGGGTCGCATTAAACAA
GTTATCGAGCAATTGACACCAGACTATGTGATTATTGATTCGATTCAGACAATGGTTCATCCGGAAACGGAAAGTGCTGC
AGGAAGTGTCAGTCAAGTGCGTGCTAACACTGCTGAATTAATGCAAATAGCTAAGACTAATAATATTGCCATTTTTATTG
TAGGGCATGTGACAAAAGAAGGCTCGCTTGCTGGACCAAGAATGTTAGAGCACATGGTTGATACAGTACTTTATTTTGAA
GGAGATAGACATCATACATTTAGAATATTGCGTGCTGTAAAAAATCGTTTTGGTTCAACGAATGAAATAGGTATTTTTGA
AATGAGACAAGCTGGTTTAGTGGAAGTGTTAAATCCATCTGAAGCTTTCTTGGAAGAGAGAATTGATGGGGCAACTGGTT
CAGCGATTGTCTGCTCAATGGAAGGAACGCGACCTATCTTAGCTGAAATTCAATCACTTGTAACGCCATCTGTTTTTGGA
AATGCGAAACGAACAGCAACAGGTCTTGACTATAATCGTGTTTCACTGATTATGGCTGTATTAGAAAAACGTGCCGGATT
ATTGTTACAAAATCAAGATGCTTATCTGAAGGCTGTGGGGGGCGTAAAACTGGATGAGCCAGCAATTGATTTATCAATTG
CAGTTAGTATTGCCTCAAGTTATAAAGATAAAGGCACATCTCCACAGGACTGTTTCATCGGTGAAATTGGGTTGACTGGT
GAAATTAGACGAGTGAATCGTATAGAGCAGCGTGTAGGAGAAGCACAGAAATTAGGATTTAGACGAGTCTTTATTCCTAT
TAATAATCTACAAGGGTGGACACCACCAACTGGAATTGAAGTGATTGGTGTCGAGACACTATCTGAAACATTATTTAAAG
TATTTTCCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(60-221)

Subunit ChlI of Mg-chelatase (ChlI)

(366-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

73.246

100

0.732
  radA Streptococcus mitis NCTC 12261

73.246

100

0.732
  radA Streptococcus pneumoniae Rx1

73.026

100

0.73
  radA Streptococcus pneumoniae D39

73.026

100

0.73
  radA Streptococcus pneumoniae R6

73.026

100

0.73
  radA Streptococcus pneumoniae TIGR4

73.026

100

0.73
  radA Bacillus subtilis subsp. subtilis str. 168

66.446

99.342

0.66