Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NR793_RS11365 Genome accession   NZ_CP102434
Coordinates   629169..629588 (-) Length   139 a.a.
NCBI ID   WP_005479693.1    Uniprot ID   Q87LT8
Organism   Vibrio parahaemolyticus strain RMDVP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624169..634588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR793_RS11345 (NR793_11370) coaE 624678..625292 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  NR793_RS11350 (NR793_11375) pilD 625293..626162 (-) 870 WP_005490359.1 A24 family peptidase Machinery gene
  NR793_RS11355 (NR793_11380) pilC 626227..627450 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  NR793_RS11360 (NR793_11385) pilB 627474..629159 (-) 1686 WP_005479695.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NR793_RS11365 (NR793_11390) pilA 629169..629588 (-) 420 WP_005479693.1 pilin Machinery gene
  NR793_RS11370 (NR793_11395) nadC 629851..630738 (-) 888 WP_005479699.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NR793_RS11375 (NR793_11400) ampD 630831..631382 (+) 552 WP_005479703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NR793_RS11380 (NR793_11405) pdhR 631788..632555 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14180.19 Da        Isoelectric Point: 4.4684

>NTDB_id=716970 NR793_RS11365 WP_005479693.1 629169..629588(-) (pilA) [Vibrio parahaemolyticus strain RMDVP1]
MKHSKQKKQQGFTLIELMIVVAIIGVLAAAAIPAYQNYVTRSEVTSGLATVKALITPAELHYQENGIAAAATLAQLGVDV
AANDLGAIDSALAGGSPTLTFTFDADSSIDGGILTFTRDAATGWSCLPTAPAIAVDGCS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=716970 NR793_RS11365 WP_005479693.1 629169..629588(-) (pilA) [Vibrio parahaemolyticus strain RMDVP1]
ATGAAACACAGTAAACAGAAAAAACAACAGGGTTTCACTCTGATTGAGTTAATGATTGTGGTAGCGATTATCGGCGTTTT
GGCTGCAGCTGCTATCCCTGCTTATCAAAATTATGTGACTCGCTCTGAAGTTACATCAGGGTTAGCAACCGTTAAAGCTC
TGATCACACCGGCTGAGCTGCATTACCAAGAAAATGGTATCGCGGCGGCTGCAACATTAGCCCAATTGGGAGTTGATGTT
GCAGCTAATGACTTAGGAGCTATTGATTCTGCCCTTGCAGGTGGTAGTCCAACTCTGACATTTACCTTCGATGCAGACAG
TTCTATCGACGGTGGGATACTAACGTTCACAAGAGACGCTGCAACTGGTTGGTCATGTTTACCTACTGCACCAGCAATTG
CAGTCGATGGTTGCAGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-129)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

100

100

1
  pilA Pseudomonas aeruginosa PAK

48.507

96.403

0.468
  pilA Vibrio cholerae strain A1552

41.892

100

0.446
  pilA Vibrio cholerae C6706

41.892

100

0.446
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.892

100

0.446
  pilA/pilAI Pseudomonas stutzeri DSM 10701

35.862

100

0.374
  comF Acinetobacter baylyi ADP1

36.691

100

0.367