Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NRF19_RS13145 Genome accession   NZ_CP102377
Coordinates   2314442..2315875 (-) Length   477 a.a.
NCBI ID   WP_103177192.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain M17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2309442..2320875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRF19_RS13115 (NRF19_13085) elbB 2311017..2311670 (+) 654 WP_327061261.1 isoprenoid biosynthesis glyoxalase ElbB -
  NRF19_RS13120 (NRF19_13090) mtgA 2311667..2312392 (+) 726 WP_327061262.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NRF19_RS13125 (NRF19_13095) npr 2312389..2312661 (-) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  NRF19_RS13130 (NRF19_13100) rapZ 2312658..2313512 (-) 855 WP_327061263.1 RNase adapter RapZ -
  NRF19_RS13135 (NRF19_13105) ptsN 2313558..2314049 (-) 492 WP_103177194.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NRF19_RS13140 (NRF19_13110) hpf 2314132..2314419 (-) 288 WP_103177193.1 ribosome hibernation promoting factor -
  NRF19_RS13145 (NRF19_13115) rpoN/rpoN1 2314442..2315875 (-) 1434 WP_103177192.1 RNA polymerase factor sigma-54 Machinery gene
  NRF19_RS13150 (NRF19_13120) lptB 2315923..2316648 (-) 726 WP_114385666.1 LPS export ABC transporter ATP-binding protein -
  NRF19_RS13155 (NRF19_13125) lptA 2316655..2317209 (-) 555 WP_103177190.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NRF19_RS13160 (NRF19_13130) lptC 2317178..2317753 (-) 576 WP_103824936.1 LPS export ABC transporter periplasmic protein LptC -
  NRF19_RS13165 (NRF19_13135) kdsC 2317750..2318316 (-) 567 WP_103824984.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NRF19_RS13170 (NRF19_13140) kdsD 2318331..2319317 (-) 987 WP_103177188.1 arabinose-5-phosphate isomerase KdsD -
  NRF19_RS13175 (NRF19_13145) - 2319331..2320308 (-) 978 WP_103824934.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54232.08 Da        Isoelectric Point: 4.3230

>NTDB_id=716635 NRF19_RS13145 WP_103177192.1 2314442..2315875(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain M17]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVETQQSQDNETLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDD
ILESIGDDEIELEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIISEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=716635 NRF19_RS13145 WP_103177192.1 2314442..2315875(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain M17]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCTATTCGTCTGTTGCA
ACTGTCGACGCTGGAACTGCAGCAGGAGCTCCAGCAAGCGCTGGAAAGTAACCCCCTGCTCGAGCAAACCGATCTTCACG
ATGAGGTAGAAACGCAACAAAGCCAGGATAACGAGACCCTCGACACGGTTGATGCCCTTGAGCAAAAAGAGATGCCTGAC
GAGCTCCCTCTCGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCAGACTACCAGGATGA
CGAGCTGCCGGTCTATCAGGGCGAAACCACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCGTTCT
CCGATACCGATCGCGCCATCGCCACCTCCATTGTGGATGCCGTGGATGAGACCGGCTACCTGACCGTGTCGCTGGACGAT
ATTCTGGAGAGCATAGGCGATGACGAGATTGAGCTCGAAGAGGTCGAAGCGGTCCTGAAACGCGTTCAGCGCTTTGACCC
GATTGGCGTCGCCGCGAGAGATCTCCGTGACTGCCTGCTCATTCAGCTTTCGCAGTTTGTTAAAGAGACCCCGTGGCTGG
AAGAGGCCCGGCTTATCATCAGCGAACATCTGGATCTGCTTGCCAACCACGATTTCCGCTCACTGATGCGCGTCACGCGG
CTGAAAGAGGAGATCCTGAAAGAGGCGGTCAACCTGATTCAGTCTCTGGATCCGCGCCCGGGGCAATCTATCCAGACCAG
CGAGCCGGAGTATGTCATTCCCGATGTGCTGGTGCGCAAGCATAACGGTCTGTGGACCGTCGAGCTGAATTCCGACAGCA
TTCCCCGCCTGCAAATCAATCAGCAATACGCCTCAATGTGCACCAGCTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGTAATCTGCAGGAAGCCCGCTGGCTGATCAAAAGCCTTGAGAGCCGCAATGATACACTGCTACGCGTCAGCCGCTGCAT
CGTCGAGCAGCAGCAGGCGTTTTTTGAGCAGGGTGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAGTCGACCATTTCTCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGTGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCGCTGAGTGACAGTAAGTTGACCACCATGCTGTCCGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.525

100

0.491