Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NRF13_RS01860 Genome accession   NZ_CP102350
Coordinates   328004..328441 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain PMC206     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 323004..333441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRF13_RS01820 (NRF13_01820) - 323582..323746 (-) 165 Protein_301 molybdopterin dinucleotide binding domain-containing protein -
  NRF13_RS01825 (NRF13_01825) - 323860..325062 (+) 1203 WP_057080005.1 GTP-binding protein -
  NRF13_RS01830 (NRF13_01830) - 325128..326264 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  NRF13_RS01835 (NRF13_01835) - 326279..326713 (+) 435 WP_058906550.1 RDD family protein -
  NRF13_RS01840 (NRF13_01840) - 326830..327105 (+) 276 WP_371745793.1 YckD family protein -
  NRF13_RS01845 (NRF13_01845) - 327209..327325 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  NRF13_RS01850 (NRF13_01850) - 327324..327543 (+) 220 Protein_307 family 1 glycosylhydrolase -
  NRF13_RS01855 (NRF13_01855) nin/comJ 327584..327982 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  NRF13_RS01860 (NRF13_01860) nucA/comI 328004..328441 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NRF13_RS01865 (NRF13_01865) hxlB 328800..329357 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  NRF13_RS01870 (NRF13_01870) hxlA 329354..329989 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  NRF13_RS01875 (NRF13_01875) - 330221..330583 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=716309 NRF13_RS01860 WP_003156588.1 328004..328441(-) (nucA/comI) [Bacillus velezensis strain PMC206]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=716309 NRF13_RS01860 WP_003156588.1 328004..328441(-) (nucA/comI) [Bacillus velezensis strain PMC206]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641