Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NL424_RS12500 Genome accession   NZ_CP117020
Coordinates   2504945..2506378 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain MLI106K3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2499945..2511378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL424_RS12470 (NL424_012465) yrbG 2500503..2501480 (+) 978 WP_001551707.1 calcium/sodium antiporter -
  NL424_RS12475 (NL424_012470) kdsD 2501494..2502480 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  NL424_RS12480 (NL424_012475) kdsC 2502501..2503067 (+) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NL424_RS12485 (NL424_012480) lptC 2503064..2503639 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  NL424_RS12490 (NL424_012485) lptA 2503608..2504165 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NL424_RS12495 (NL424_012490) lptB 2504172..2504897 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  NL424_RS12500 (NL424_012495) rpoN/rpoN1 2504945..2506378 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  NL424_RS12505 (NL424_012500) hpf 2506401..2506688 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  NL424_RS12510 (NL424_012505) ptsN 2506806..2507297 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NL424_RS12515 (NL424_012510) rapZ 2507343..2508197 (+) 855 WP_000243741.1 RNase adapter RapZ -
  NL424_RS12520 (NL424_012515) npr 2508194..2508466 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  NL424_RS12525 (NL424_012520) yrbL 2508679..2509311 (+) 633 WP_000620396.1 PhoP regulatory network protein YrbL -
  NL424_RS12530 (NL424_012525) mtgA 2509308..2510036 (-) 729 WP_085018144.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NL424_RS12535 (NL424_012530) elbB 2510033..2510686 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=716255 NL424_RS12500 WP_000809051.1 2504945..2506378(+) (rpoN/rpoN1) [Escherichia coli strain MLI106K3]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=716255 NL424_RS12500 WP_000809051.1 2504945..2506378(+) (rpoN/rpoN1) [Escherichia coli strain MLI106K3]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CTGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCACTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCAGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTTAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465