Detailed information NTG0716223

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: NQK75_RS14985
• Genome accession: NZ_CP102329
• Coordinates: 3208394..3209548 (+)
• Length: 384 a.a.
• NCBI ID: WP_021163045.1
• Uniprot ID: U2H1M2
• Organism: Burkholderia cepacia strain N3009-2YT
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3203394..3214548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NQK75_RS14955	-	3204024..3204629 (+)	606	WP_021163051.1	50S ribosomal protein L25/general stress protein Ctc	-
NQK75_RS14960	pth	3204762..3205361 (+)	600	WP_192386538.1	aminoacyl-tRNA hydrolase	-
NQK75_RS14965	hisC	3205476..3206543 (+)	1068	WP_192386540.1	histidinol-phosphate transaminase	-
NQK75_RS14970	-	3206628..3206894 (-)	267	WP_006477774.1	YfhL family 4Fe-4S dicluster ferredoxin	-
NQK75_RS14975	coaD	3206954..3207451 (-)	498	WP_021163047.1	pantetheine-phosphate adenylyltransferase	-
NQK75_RS14980	rsmD	3207563..3208177 (-)	615	WP_048025675.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
NQK75_RS14985	pilA	3208394..3209548 (+)	1155	WP_021163045.1	signal recognition particle-docking protein FtsY	Machinery gene
NQK75_RS14990	maiA	3209632..3210276 (-)	645	WP_059498604.1	maleylacetoacetate isomerase	-
NQK75_RS14995	ybiB	3210470..3211443 (+)	974	Protein_2928	DNA-binding protein YbiB	-
NQK75_RS15000	leuA	3211824..3213470 (+)	1647	WP_060129696.1	2-isopropylmalate synthase	-
NQK75_RS15005	-	3213611..3213994 (-)	384	WP_027789089.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 40898.82 Da
• Isoelectric Point: 4.8018

>NTDB_id=716223 NQK75_RS14985 WP_021163045.1 3208394..3209548(+) (pilA) [Burkholderia cepacia strain N3009-2YT]
MFSFFKRFKKTQEPDPAESQSADAQQTDEPSDAPAVEAPPAAEVPQAPAQPAAPAVVMTVTPTNDGRDEVVETVEIVPPP
LQDATAKKSWLARLKTGLAKTGSSITGVFVNTKIDEDLYEELETALLMSDAGVDATEYLLGALREKVRAGRLTDPQQVKA
ALHDLLVELLTPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERNNV
TVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQNALTQ
VKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAVEFADALLG

Nucleotide

• Length: 1155 bp

>NTDB_id=716223 NQK75_RS14985 WP_021163045.1 3208394..3209548(+) (pilA) [Burkholderia cepacia strain N3009-2YT]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGATCCGGCGGAATCGCAATCGGCCGACGCGCAGCAAAC
GGACGAACCGTCCGATGCGCCCGCGGTCGAGGCCCCGCCTGCGGCCGAGGTGCCGCAAGCGCCCGCGCAGCCGGCCGCGC
CGGCCGTCGTGATGACGGTCACGCCGACCAACGACGGGCGCGACGAAGTCGTCGAGACGGTCGAAATCGTTCCGCCGCCG
CTGCAGGACGCCACCGCGAAGAAATCGTGGCTCGCCCGCCTGAAAACGGGGCTCGCCAAGACGGGCTCGAGCATCACCGG
CGTCTTCGTCAACACGAAGATCGACGAGGATCTGTACGAGGAGCTCGAAACCGCGCTGCTGATGTCCGACGCGGGCGTCG
ACGCGACCGAGTACCTGCTCGGCGCGCTACGCGAAAAGGTGCGCGCGGGCCGCCTGACCGACCCGCAGCAGGTGAAGGCC
GCGCTGCACGACCTGCTCGTCGAGTTGCTGACGCCGCTCGAGAAGTCGCTGATGCTCGGCCGCGCGCAGCCGCTCGTGAT
GATGATCACCGGCGTGAACGGTGCGGGCAAGACGACCAGCATCGGCAAGCTCGCAAAGCATCTGCAGAGCTTCGACCAGT
CGGTGCTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAACAACGTG
ACGGTCGTGCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAAGATCGA
CGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACACAGCTCCACCTGATGGAAGAGCTGAAGAAGGTGAAGCGCGTGA
TCTCGAAGGCGCACGACGGCGCGCCGCACGAAGTGCTGCTGGTGATCGACGCGAACACCGGACAGAACGCGCTCACGCAG
GTCAAGGCATTCGACGACGCGCTCGGCCTCACCGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGGATTCT
CGCCGCGATCGCGCGGCAGCGCCCGGTGCCGGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGATCTGCAGCCGTTCA
GCGCGGTCGAATTCGCAGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (102-164)

• SRP54-type protein, GTPase domain (SRP54) (185-384)

3D structure

• AlphaFold DB: U2H1M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.846	100	0.583