Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NQK75_RS14890 Genome accession   NZ_CP102329
Coordinates   3191727..3193232 (+) Length   501 a.a.
NCBI ID   WP_042977154.1    Uniprot ID   -
Organism   Burkholderia cepacia strain N3009-2YT     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3186727..3198232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQK75_RS14865 kdsD 3187959..3188942 (+) 984 WP_021162595.1 arabinose 5-phosphate isomerase KdsD -
  NQK75_RS14870 - 3188942..3189478 (+) 537 WP_021162596.1 HAD family hydrolase -
  NQK75_RS14875 lptC 3189482..3190096 (+) 615 WP_021162597.1 LPS export ABC transporter periplasmic protein LptC -
  NQK75_RS14880 lptA 3190117..3190782 (+) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  NQK75_RS14885 lptB 3190779..3191555 (+) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  NQK75_RS14890 rpoN/rpoN1 3191727..3193232 (+) 1506 WP_042977154.1 RNA polymerase factor sigma-54 Machinery gene
  NQK75_RS14895 hpf 3193391..3193750 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NQK75_RS14900 - 3193920..3194459 (+) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  NQK75_RS14905 hprK 3194591..3195559 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  NQK75_RS14910 rapZ 3195635..3196543 (+) 909 WP_021162602.1 RNase adapter RapZ -
  NQK75_RS14915 - 3196571..3197206 (+) 636 WP_021162603.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54827.96 Da        Isoelectric Point: 4.8767

>NTDB_id=716222 NQK75_RS14890 WP_042977154.1 3191727..3193232(+) (rpoN/rpoN1) [Burkholderia cepacia strain N3009-2YT]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=716222 NQK75_RS14890 WP_042977154.1 3191727..3193232(+) (rpoN/rpoN1) [Burkholderia cepacia strain N3009-2YT]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGATGAATGGA
TCGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCGCTGCG
ACAAACAGTAGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGTTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGTGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.245

100

0.691