Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NRK99_RS20455 Genome accession   NZ_CP102326
Coordinates   4436869..4438308 (-) Length   479 a.a.
NCBI ID   WP_126880648.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC04     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4431869..4443308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK99_RS20425 (NRK99_20425) dagF 4432145..4432885 (+) 741 WP_126880646.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  NRK99_RS20430 (NRK99_20430) - 4432989..4434893 (+) 1905 WP_126880647.1 BglG family transcription antiterminator -
  NRK99_RS20435 (NRK99_20435) npr 4434909..4435181 (-) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  NRK99_RS20440 (NRK99_20440) rapZ 4435202..4436068 (-) 867 WP_005305946.1 RNase adapter RapZ -
  NRK99_RS20445 (NRK99_20445) ptsN 4436111..4436557 (-) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NRK99_RS20450 (NRK99_20450) hpf 4436560..4436847 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  NRK99_RS20455 (NRK99_20455) rpoN/rpoN1 4436869..4438308 (-) 1440 WP_126880648.1 RNA polymerase factor sigma-54 Machinery gene
  NRK99_RS20460 (NRK99_20460) lptB 4438374..4439099 (-) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  NRK99_RS20465 (NRK99_20465) lptA 4439103..4439630 (-) 528 WP_010635928.1 lipopolysaccharide transport periplasmic protein LptA -
  NRK99_RS20470 (NRK99_20470) lptC 4439617..4440177 (-) 561 WP_010635927.1 LPS export ABC transporter periplasmic protein LptC -
  NRK99_RS20475 (NRK99_20475) kdsC 4440174..4440728 (-) 555 WP_017782403.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NRK99_RS20480 (NRK99_20480) - 4440728..4441723 (-) 996 WP_268000082.1 KpsF/GutQ family sugar-phosphate isomerase -
  NRK99_RS20485 (NRK99_20485) mlaF 4441931..4442731 (+) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53862.81 Da        Isoelectric Point: 4.3584

>NTDB_id=716150 NRK99_RS20455 WP_126880648.1 4436869..4438308(-) (rpoN/rpoN1) [Aeromonas dhakensis strain BC04]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLRENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=716150 NRK99_RS20455 WP_126880648.1 4436869..4438308(-) (rpoN/rpoN1) [Aeromonas dhakensis strain BC04]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTACTCGGCCGGCACCGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCCATCGACGAATCCGGTTACCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCCCCTGACTTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTCGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTGAGAGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCACGGCCCGGTAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCGG
ACTCGGCCCCGCGCATCCGGGTCAACGAAACCTATGCTGCCATGGCCAGACAGGCCCGCACCAGTCAGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGCCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGTTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACCACCCAGAAGTACATGCACACGCCGCGCGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486