Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   NL414_RS25555 Genome accession   NZ_CP117013
Coordinates   5144941..5146770 (-) Length   609 a.a.
NCBI ID   WP_024185236.1    Uniprot ID   -
Organism   Escherichia coli strain MLI114     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5139941..5151770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL414_RS25530 (NL414_025530) bioP 5140659..5141558 (-) 900 WP_001196244.1 biotin transporter -
  NL414_RS25535 (NL414_025535) yigL 5141634..5142434 (-) 801 WP_000285353.1 sugar/pyridoxal phosphate phosphatase YigL -
  NL414_RS25540 (NL414_025540) pldB 5142442..5143464 (-) 1023 WP_000487671.1 lysophospholipase L2 -
  NL414_RS25545 (NL414_025545) rhtB 5143575..5144195 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  NL414_RS25550 (NL414_025550) rhtC 5144257..5144877 (-) 621 WP_000928844.1 threonine export protein RhtC -
  NL414_RS25555 (NL414_025555) recQ 5144941..5146770 (-) 1830 WP_024185236.1 ATP-dependent DNA helicase RecQ Machinery gene
  NL414_RS25560 (NL414_025560) pldA 5146895..5147764 (-) 870 WP_001259693.1 phospholipase A -
  NL414_RS25565 (NL414_025565) yigI 5147929..5148396 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  NL414_RS25570 (NL414_025570) rarD 5148448..5149338 (+) 891 WP_000339111.1 EamA family transporter RarD -
  NL414_RS25575 (NL414_025575) - 5149648..5150496 (+) 849 WP_001059673.1 YPO3983 family protein -
  NL414_RS25580 (NL414_025580) - 5150493..5150990 (+) 498 WP_000837551.1 DUF943 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68324.48 Da        Isoelectric Point: 7.0847

>NTDB_id=716129 NL414_RS25555 WP_024185236.1 5144941..5146770(-) (recQ) [Escherichia coli strain MLI114]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCCTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNIRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=716129 NL414_RS25555 WP_024185236.1 5144941..5146770(-) (recQ) [Escherichia coli strain MLI114]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCTTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCTGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGGCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATATTCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAACAAGCCTAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTACGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAATTGCAGGATATCGAGCGCCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGGGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCACGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCCTTCGGCGGCAACTACGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGTGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389