Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NRK98_RS20670 Genome accession   NZ_CP102325
Coordinates   4548723..4550162 (+) Length   479 a.a.
NCBI ID   WP_010635929.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4543723..4555162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK98_RS20640 (NRK98_20640) mlaF 4544300..4545100 (-) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -
  NRK98_RS20645 (NRK98_20645) - 4545308..4546303 (+) 996 WP_005305976.1 KpsF/GutQ family sugar-phosphate isomerase -
  NRK98_RS20650 (NRK98_20650) kdsC 4546303..4546857 (+) 555 WP_017766397.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NRK98_RS20655 (NRK98_20655) lptC 4546854..4547414 (+) 561 WP_010635927.1 LPS export ABC transporter periplasmic protein LptC -
  NRK98_RS20660 (NRK98_20660) lptA 4547401..4547928 (+) 528 WP_099359776.1 lipopolysaccharide transport periplasmic protein LptA -
  NRK98_RS20665 (NRK98_20665) lptB 4547932..4548657 (+) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  NRK98_RS20670 (NRK98_20670) rpoN/rpoN1 4548723..4550162 (+) 1440 WP_010635929.1 RNA polymerase factor sigma-54 Machinery gene
  NRK98_RS20675 (NRK98_20675) hpf 4550184..4550471 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  NRK98_RS20680 (NRK98_20680) ptsN 4550474..4550920 (+) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NRK98_RS20685 (NRK98_20685) rapZ 4550963..4551829 (+) 867 WP_005305946.1 RNase adapter RapZ -
  NRK98_RS20690 (NRK98_20690) npr 4551850..4552122 (+) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  NRK98_RS20695 (NRK98_20695) - 4552138..4554042 (-) 1905 WP_268027955.1 BglG family transcription antiterminator -
  NRK98_RS20700 (NRK98_20700) dagF 4554147..4554887 (-) 741 WP_206865942.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53848.82 Da        Isoelectric Point: 4.3584

>NTDB_id=716122 NRK98_RS20670 WP_010635929.1 4548723..4550162(+) (rpoN/rpoN1) [Aeromonas dhakensis strain BC03]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLKENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=716122 NRK98_RS20670 WP_010635929.1 4548723..4550162(+) (rpoN/rpoN1) [Aeromonas dhakensis strain BC03]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTATTCGGCCGGCACGGCTCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCATCTATCAGGGTGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCCATCGACGAATCCGGTTATCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCACCTGATCTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTTGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCTTGGCCCGCAAG
ACCAAGCTGAAGGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGCGGCCCGGCAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCGGCCCCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCAGACAGGCTCGCACCAGTCAGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACAATCTCGCGGGTGACTACCCAGAAGTACATGCACACGCCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486