Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRL03_RS18980 Genome accession   NZ_CP102315
Coordinates   4062921..4064627 (+) Length   568 a.a.
NCBI ID   WP_337984053.1    Uniprot ID   -
Organism   Aeromonas caviae strain BC01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4057921..4069627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRL03_RS18955 (NRL03_18985) pdhR 4059102..4059866 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NRL03_RS18960 (NRL03_18990) ampD 4060143..4060715 (-) 573 WP_337984050.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRL03_RS18965 (NRL03_18995) - 4060844..4061314 (+) 471 WP_102949049.1 retropepsin-like aspartic protease family protein -
  NRL03_RS18970 (NRL03_19000) nadC 4061318..4062181 (+) 864 WP_337984051.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRL03_RS18975 (NRL03_19005) - 4062378..4062917 (+) 540 WP_337984052.1 pilin -
  NRL03_RS18980 (NRL03_19010) pilB 4062921..4064627 (+) 1707 WP_337984053.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRL03_RS18985 (NRL03_19015) pilC 4064755..4065996 (+) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  NRL03_RS18990 (NRL03_19020) pilD 4066074..4066949 (+) 876 WP_052814465.1 prepilin peptidase Machinery gene
  NRL03_RS18995 (NRL03_19025) coaE 4066968..4067582 (+) 615 WP_052814464.1 dephospho-CoA kinase -
  NRL03_RS19000 (NRL03_19030) zapD 4067620..4068342 (+) 723 WP_010675825.1 cell division protein ZapD -
  NRL03_RS19005 (NRL03_19035) yacG 4068353..4068547 (+) 195 WP_103858092.1 DNA gyrase inhibitor YacG -
  NRL03_RS19010 (NRL03_19040) mutT 4068605..4069009 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62572.96 Da        Isoelectric Point: 5.9400

>NTDB_id=716064 NRL03_RS18980 WP_337984053.1 4062921..4064627(+) (pilB) [Aeromonas caviae strain BC01]
MTSSPHSGLASSLAASSLLSEPDSLRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716064 NRL03_RS18980 WP_337984053.1 4062921..4064627(+) (pilB) [Aeromonas caviae strain BC01]
ATGACCTCTAGCCCCCATAGCGGCCTGGCCTCCAGCCTGGCCGCCTCTTCCCTGCTTAGTGAGCCGGACTCACTGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTTTTGATTGAGAACGATATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAACTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATCGAGAAGCACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCATACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGTGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAGCTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGCTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCGGGCAAGACGGTGTCACTCTATACCGGCCTCAACATCCTCAATACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTTGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
TTGGAAACCGCCGAGATTGCTATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAGAAGGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.674

99.824

0.546
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Acinetobacter baylyi ADP1

54.401

100

0.544
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

50.177

99.648

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

50

98.944

0.495
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37