Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NQ541_RS02835 Genome accession   NZ_CP102292
Coordinates   576573..577937 (+) Length   454 a.a.
NCBI ID   WP_005608905.1    Uniprot ID   B5CSY0
Organism   [Ruminococcus] lactaris ATCC 29176     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 571573..582937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ541_RS02815 (NQ541_02815) - 571724..573076 (+) 1353 WP_005608912.1 sensor histidine kinase -
  NQ541_RS02820 (NQ541_02820) - 573164..573532 (+) 369 WP_005608910.1 GntR family transcriptional regulator -
  NQ541_RS02825 (NQ541_02825) - 573558..575996 (+) 2439 WP_005608908.1 ATP-dependent Clp protease ATP-binding subunit -
  NQ541_RS02830 (NQ541_02830) - 576126..576539 (+) 414 WP_005608907.1 hypothetical protein -
  NQ541_RS02835 (NQ541_02835) radA 576573..577937 (+) 1365 WP_005608905.1 DNA repair protein RadA Machinery gene
  NQ541_RS02840 (NQ541_02840) - 578261..578632 (+) 372 WP_023922074.1 GntR family transcriptional regulator -
  NQ541_RS02845 (NQ541_02845) - 578635..579486 (+) 852 WP_005608898.1 ATP-binding cassette domain-containing protein -
  NQ541_RS02850 (NQ541_02850) - 579483..580175 (+) 693 WP_005608896.1 ABC-2 transporter permease -
  NQ541_RS02855 (NQ541_02855) - 580381..580824 (+) 444 WP_005608894.1 MarR family winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49398.32 Da        Isoelectric Point: 6.9060

>NTDB_id=715961 NQ541_RS02835 WP_005608905.1 576573..577937(+) (radA) [[Ruminococcus] lactaris ATCC 29176]
MAKGKKSIFFCQNCGHEEAKWLGQCPACKEWNTFVEERIDSGITKGTTVAARAVHEAKVVPLTEVTADDDTRSETGIKEL
DRVLGGGIVPGSLVLVGGDPGIGKSTLLLQVCQRMAQMKKILYISGEESQAQIKLRANRMGNFTSGLLLLCETNLGIIRS
VIEKERPELVVIDSIQTMYSEDVTSAPGSVSQVRESTNVFMQLAKGLCIPIFIVGHVTKEGTVAGPRVLEHMVDTVLYFE
GDRHASYRILRAVKNRFGSTNEIGVFEMRQSGLVEVENPSEYMLSGKPENASGSVVACSMEGTRPILLEIQALVCRTNFG
MPRRTAAGTDYNRVNLLMAVLEKRLGMSLGNCDAYVNIAGGIKMNEPAIDLGIVMALVSSYRNRPIDEKTIVFGEVGLSG
EVRAVNMPEQRVAEAKKLGFETCILPEVSLKMVKEIQGIRLVGVKTVNEAVGII

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=715961 NQ541_RS02835 WP_005608905.1 576573..577937(+) (radA) [[Ruminococcus] lactaris ATCC 29176]
ATGGCAAAAGGAAAGAAAAGTATATTTTTCTGTCAGAATTGCGGGCATGAAGAAGCAAAATGGCTTGGTCAATGCCCTGC
ATGTAAAGAGTGGAATACATTTGTAGAAGAGCGGATAGACAGTGGGATCACAAAGGGAACGACCGTTGCAGCGAGAGCAG
TTCACGAGGCAAAGGTCGTTCCTTTGACCGAAGTGACCGCAGATGATGATACACGGAGTGAGACCGGGATCAAAGAGCTG
GACCGGGTTCTCGGAGGCGGGATTGTACCGGGATCACTGGTACTGGTCGGAGGCGATCCGGGAATTGGAAAGTCAACACT
TTTATTACAGGTATGCCAAAGAATGGCACAGATGAAAAAGATCCTGTATATTTCCGGAGAGGAGTCGCAGGCACAGATTA
AGCTGCGTGCGAACCGGATGGGAAATTTTACGTCTGGCCTTCTGCTGCTTTGCGAGACAAACCTGGGGATCATCCGCAGT
GTGATAGAAAAAGAGCGACCGGAACTGGTCGTTATTGATTCTATTCAGACAATGTACAGCGAGGATGTAACTTCGGCACC
GGGAAGTGTTTCCCAGGTGCGGGAGTCAACCAATGTTTTTATGCAGTTGGCGAAAGGACTGTGCATTCCAATTTTTATTG
TCGGACATGTAACGAAAGAGGGAACGGTAGCAGGTCCGCGGGTATTGGAGCATATGGTAGATACAGTACTTTATTTTGAA
GGAGACCGGCATGCGTCTTACCGGATATTGAGAGCTGTGAAGAACCGTTTTGGCTCGACGAATGAGATTGGTGTCTTTGA
AATGCGGCAGAGTGGGTTGGTGGAAGTGGAAAATCCATCGGAGTATATGCTCAGTGGGAAACCGGAGAATGCTTCCGGGT
CCGTTGTTGCATGCTCCATGGAAGGAACAAGACCGATCCTTTTGGAAATACAGGCACTGGTCTGCAGAACAAATTTCGGA
ATGCCGAGAAGAACAGCAGCAGGAACCGATTATAACCGGGTCAACCTTCTGATGGCAGTGCTTGAAAAGAGGTTGGGAAT
GTCACTTGGAAACTGCGATGCATATGTGAATATTGCAGGCGGTATCAAGATGAACGAGCCGGCGATCGATCTTGGGATTG
TGATGGCACTGGTGTCCAGTTATCGAAACCGTCCCATTGATGAGAAGACGATTGTCTTTGGAGAGGTTGGTTTAAGTGGA
GAAGTACGTGCTGTGAATATGCCGGAACAGCGTGTGGCAGAGGCGAAGAAGCTTGGGTTTGAGACATGTATCTTGCCCGA
AGTTTCACTGAAGATGGTAAAGGAAATTCAGGGAATACGGCTTGTTGGTGTGAAGACTGTGAATGAAGCAGTTGGAATCA
TATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-36)

Subunit ChlI of Mg-chelatase (ChlI)

(345-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B5CSY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

56.512

99.78

0.564
  radA Streptococcus mitis NCTC 12261

54.545

99.339

0.542
  radA Streptococcus pneumoniae Rx1

54.545

99.339

0.542
  radA Streptococcus pneumoniae D39

54.545

99.339

0.542
  radA Streptococcus pneumoniae R6

54.545

99.339

0.542
  radA Streptococcus pneumoniae TIGR4

54.545

99.339

0.542
  radA Streptococcus mitis SK321

54.545

99.339

0.542