Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   NQ504_RS04285 Genome accession   NZ_CP102284
Coordinates   849430..851235 (+) Length   601 a.a.
NCBI ID   WP_003697942.1    Uniprot ID   E7FNK8
Organism   Ligilactobacillus ruminis strain ATCC 25644     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 844430..856235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ504_RS04260 (NQ504_04260) - 845158..845988 (+) 831 WP_259962340.1 IS3 family transposase -
  NQ504_RS04265 (NQ504_04265) - 846109..846669 (-) 561 WP_003693554.1 M15 family metallopeptidase -
  NQ504_RS04270 (NQ504_04270) spxA 846984..847382 (+) 399 WP_022280671.1 transcriptional regulator SpxA -
  NQ504_RS04275 (NQ504_04275) - 847495..848271 (+) 777 WP_003697940.1 adaptor protein MecA -
  NQ504_RS04280 (NQ504_04280) - 848421..849347 (+) 927 WP_004563619.1 competence protein CoiA -
  NQ504_RS04285 (NQ504_04285) pepF 849430..851235 (+) 1806 WP_003697942.1 oligoendopeptidase F Regulator
  NQ504_RS04290 (NQ504_04290) - 851276..851923 (-) 648 WP_003693563.1 ClpXP adapter SpxH family protein -
  NQ504_RS04295 (NQ504_04295) - 852249..852938 (+) 690 WP_003697945.1 GTP pyrophosphokinase family protein -
  NQ504_RS04300 (NQ504_04300) - 852935..853741 (+) 807 WP_003693569.1 NAD kinase -
  NQ504_RS04305 (NQ504_04305) - 853731..854636 (+) 906 WP_003697946.1 RluA family pseudouridine synthase -
  NQ504_RS04310 (NQ504_04310) mgtE 854642..856012 (+) 1371 WP_003693572.1 magnesium transporter -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69519.81 Da        Isoelectric Point: 4.7587

>NTDB_id=715864 NQ504_RS04285 WP_003697942.1 849430..851235(+) (pepF) [Ligilactobacillus ruminis strain ATCC 25644]
MAEVKQLPKREEVPVELTWDLTKIYATDTDFEADFAKVKKEAESFKKYQGKLRENGDTLLEAIKEMFAIQRRLENVYVYA
SMKNDQDTANAENQAKNATVQKLVAEVSEKLSWFQPELLHLTIEELEEAKKQAPELNEYSQNLREWFEMKDHVLSDKEEA
LLAGASDIFSAPESTFEVLNDADLKFPVMKDENGDDVELSEGIYGTLLESTDRRVRKEAFQNLYKVYRQFRHTFAMTLSS
HNRTENYVAKVRNYSTAQEAALANNHIPVSVYDTLINVTNKHLPLLHRYVALRKKLLNLDEVHMYDLYTPITGEPTLKYT
YDEAKEEALKALAVLGDDYLSHVKEAFDNRWIDVVANQGKRGGAYSGGSYDTAPYILLNWQDTLDNLFTLVHEMGHTIHS
YYTRHNQPYQYGDYSIFVAEIASTTNENLLTQYLLDHFEDKKVKAYVLNHYLDGFKGTVFRQTQFAEFEKWSHEQDAAGK
PLTADVISKYYGELNKRYYGPDIFNDDEISLEWARIPHFYYNYYVYQYATGFAAATTLAQNILNDNPADTQAYLTYLKSG
SSEYPIDIMKKAGVDMTNEEYLEKAFDVFEKRLNELEKLIG

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=715864 NQ504_RS04285 WP_003697942.1 849430..851235(+) (pepF) [Ligilactobacillus ruminis strain ATCC 25644]
ATGGCTGAAGTAAAGCAACTTCCAAAACGTGAAGAAGTTCCGGTTGAACTGACATGGGATCTCACGAAAATTTATGCTAC
GGATACGGACTTCGAAGCAGATTTTGCCAAAGTAAAAAAAGAAGCTGAGAGTTTCAAAAAATATCAGGGCAAGCTTAGGG
AAAACGGAGATACCTTGCTCGAAGCAATTAAAGAAATGTTTGCGATTCAAAGGCGTCTTGAGAATGTTTATGTTTATGCA
AGCATGAAGAATGATCAAGATACTGCCAATGCTGAAAATCAAGCCAAGAACGCAACCGTTCAAAAACTTGTGGCCGAAGT
TTCGGAAAAACTTTCCTGGTTTCAACCGGAGCTTTTGCATTTGACGATTGAAGAGCTTGAGGAGGCAAAGAAGCAGGCAC
CTGAACTTAACGAATATTCACAAAATCTCAGAGAATGGTTTGAGATGAAAGATCACGTCTTAAGCGACAAGGAAGAAGCA
TTGCTTGCCGGTGCGAGCGACATCTTCAGTGCACCTGAAAGCACTTTTGAAGTTTTGAACGATGCTGATTTGAAATTCCC
TGTCATGAAGGACGAAAACGGTGATGACGTTGAGCTTTCAGAAGGAATCTACGGAACGTTGCTCGAATCGACGGATCGTC
GTGTTCGCAAAGAAGCTTTCCAAAATTTGTATAAGGTTTATCGCCAGTTTCGTCATACGTTTGCTATGACGCTGTCTTCA
CACAACAGGACCGAAAATTACGTTGCCAAAGTCAGAAATTACTCAACGGCACAAGAGGCCGCTTTGGCAAACAATCACAT
CCCGGTCTCTGTTTATGATACGCTTATCAATGTAACGAATAAGCATTTGCCTTTGCTGCATCGCTACGTTGCGCTCAGGA
AGAAATTGCTCAATCTTGACGAGGTGCATATGTATGATCTGTATACGCCGATCACGGGCGAGCCGACCCTCAAATATACG
TATGATGAAGCCAAGGAAGAAGCATTAAAAGCATTGGCTGTTTTAGGTGACGATTATCTTTCTCACGTTAAAGAAGCTTT
TGACAATCGTTGGATTGACGTTGTGGCAAATCAGGGCAAGCGCGGCGGAGCGTACTCTGGCGGATCTTACGATACTGCCC
CGTACATTTTGCTCAATTGGCAGGATACGCTTGATAATTTGTTTACGCTTGTACATGAAATGGGACATACGATTCACAGC
TACTATACTCGTCATAATCAACCTTATCAATACGGGGATTATTCAATTTTTGTTGCTGAAATTGCTTCAACGACAAACGA
AAACTTATTGACGCAATATTTGCTTGATCATTTTGAAGACAAAAAAGTTAAAGCATATGTTTTAAATCACTATCTCGATG
GTTTCAAGGGAACGGTTTTCCGTCAGACGCAATTTGCTGAATTTGAAAAATGGTCCCATGAACAAGACGCTGCAGGCAAG
CCGCTGACTGCCGATGTTATCAGCAAGTACTATGGCGAGCTGAATAAGCGTTACTACGGGCCTGATATTTTTAATGACGA
TGAAATTTCGCTTGAATGGGCACGAATTCCTCATTTTTACTACAACTATTACGTCTATCAATATGCAACGGGATTTGCTG
CTGCAACGACTTTGGCACAAAATATCCTAAATGATAATCCTGCCGATACGCAAGCATATCTGACATATTTGAAGTCAGGA
TCGAGCGAATATCCAATTGACATCATGAAAAAAGCTGGCGTTGATATGACGAATGAAGAGTATCTCGAAAAAGCATTTGA
CGTCTTTGAAAAGCGACTGAATGAATTGGAGAAACTCATTGGTTGA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E7FNK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

53.976

98.336

0.531