Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   URS_RS01890 Genome accession   NZ_AP018824
Coordinates   401403..402155 (-) Length   250 a.a.
NCBI ID   WP_004994852.1    Uniprot ID   A0A3G9FKH4
Organism   Acinetobacter ursingii strain M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396403..407155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  URS_RS01870 (URS_0389) - 397460..398161 (-) 702 WP_005002273.1 tetratricopeptide repeat protein -
  URS_RS01875 (URS_0390) hisS 398191..399483 (-) 1293 WP_005002271.1 histidine--tRNA ligase -
  URS_RS01880 (URS_0391) ispG 399487..400605 (-) 1119 WP_004992682.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  URS_RS01885 (URS_0392) - 400630..401412 (-) 783 WP_004994849.1 helix-turn-helix domain-containing protein -
  URS_RS01890 (URS_0393) pilF 401403..402155 (-) 753 WP_004994852.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  URS_RS01895 (URS_0394) rlmN 402223..403449 (-) 1227 WP_119686305.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  URS_RS01905 (URS_0395) ndk 403571..404002 (-) 432 WP_004994861.1 nucleoside-diphosphate kinase -
  URS_RS01910 (URS_0396) iscX 404114..404314 (-) 201 WP_004992669.1 Fe-S cluster assembly protein IscX -
  URS_RS01915 (URS_0397) - 404419..404970 (-) 552 WP_049174902.1 phosphatase PAP2 family protein -
  URS_RS01920 (URS_0398) - 405000..406277 (-) 1278 WP_119686304.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28394.23 Da        Isoelectric Point: 9.3924

>NTDB_id=71555 URS_RS01890 WP_004994852.1 401403..402155(-) (pilF) [Acinetobacter ursingii strain M3]
MALLLTGCQSQKTTKDPKKAVQVRTQLAAEYIKSGDLDAAKRALDQALEVNSRDSMANMMMGVLLQQEGSATNLEKADTY
FKRAISEEPTNAQARNNYGTYLYQLKRYNDAIEQFTIAGATLGYDQRYRALENLGRIYVLLGDRVNAEKSFKQALQANRD
SYVSMLELSEIFYLQQQMPAATQMYEQYVRTVGQTNQGARALWVGIRVARANGDRMGMQALINQLRALFPDSSEYKRYLQ
LQYSTEAVWK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=71555 URS_RS01890 WP_004994852.1 401403..402155(-) (pilF) [Acinetobacter ursingii strain M3]
ATGGCGTTGCTGTTAACGGGATGCCAATCTCAAAAAACAACCAAAGACCCTAAAAAAGCGGTGCAGGTTCGTACACAACT
TGCAGCTGAATATATCAAGTCTGGTGATCTGGATGCTGCTAAACGCGCATTGGATCAGGCTTTGGAGGTGAACTCGCGTG
ACTCCATGGCCAATATGATGATGGGTGTACTCTTACAACAAGAAGGTAGTGCAACAAATCTCGAGAAAGCAGACACGTAT
TTTAAACGTGCAATCTCGGAAGAGCCTACCAATGCACAAGCTCGTAATAATTATGGTACGTACTTATACCAATTGAAACG
TTATAATGACGCGATTGAACAATTTACGATTGCAGGGGCAACACTTGGGTATGATCAGCGTTATCGTGCCCTTGAAAATC
TGGGGCGTATCTACGTATTGCTTGGCGATCGTGTGAATGCTGAAAAGTCATTCAAACAAGCTTTACAGGCAAACCGCGAC
TCTTATGTATCTATGTTAGAGTTGTCAGAGATTTTTTATTTGCAACAGCAAATGCCAGCGGCGACTCAAATGTATGAACA
ATATGTGCGTACAGTGGGGCAAACCAATCAGGGGGCGCGTGCACTTTGGGTGGGGATTCGTGTTGCGCGAGCCAATGGTG
ATCGAATGGGAATGCAGGCACTGATCAATCAACTGCGTGCTTTATTTCCCGATAGTTCAGAATATAAACGTTATTTGCAA
TTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(81-113)

Tetratricopeptide repeat (TPR_7)

(130-160)

Tetratricopeptide repeat (TPR_8)

(23-52)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G9FKH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

81.275

100

0.816
  pilF Acinetobacter baylyi ADP1

77.108

99.6

0.768

Multiple sequence alignment