Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NQ230_RS02950 Genome accession   NZ_CP102247
Coordinates   616784..618217 (-) Length   477 a.a.
NCBI ID   WP_063143582.1    Uniprot ID   -
Organism   Enterobacter asburiae strain R_A5.MM     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 611784..623217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ230_RS02920 (NQ230_02920) elbB 613317..613970 (+) 654 WP_257261304.1 isoprenoid biosynthesis glyoxalase ElbB -
  NQ230_RS02925 (NQ230_02925) mtgA 613967..614692 (+) 726 WP_121424779.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NQ230_RS02930 (NQ230_02930) npr 614731..615003 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  NQ230_RS02935 (NQ230_02935) rapZ 615000..615854 (-) 855 WP_014885397.1 RNase adapter RapZ -
  NQ230_RS02940 (NQ230_02940) ptsN 615900..616391 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NQ230_RS02945 (NQ230_02945) hpf 616474..616761 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  NQ230_RS02950 (NQ230_02950) rpoN/rpoN1 616784..618217 (-) 1434 WP_063143582.1 RNA polymerase factor sigma-54 Machinery gene
  NQ230_RS02955 (NQ230_02955) lptB 618265..618990 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  NQ230_RS02960 (NQ230_02960) lptA 618997..619551 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NQ230_RS02965 (NQ230_02965) lptC 619520..620095 (-) 576 WP_121424777.1 LPS export ABC transporter periplasmic protein LptC -
  NQ230_RS02970 (NQ230_02970) kdsC 620092..620658 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NQ230_RS02975 (NQ230_02975) kdsD 620673..621659 (-) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  NQ230_RS02980 (NQ230_02980) - 621673..622650 (-) 978 WP_023309354.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54197.95 Da        Isoelectric Point: 4.3173

>NTDB_id=715455 NQ230_RS02950 WP_063143582.1 616784..618217(-) (rpoN/rpoN1) [Enterobacter asburiae strain R_A5.MM]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=715455 NQ230_RS02950 WP_063143582.1 616784..618217(-) (rpoN/rpoN1) [Enterobacter asburiae strain R_A5.MM]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCTCAGGATACAGAAGCGCTTGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGAAGAGATTGAGCTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGTGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGCTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGTTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTGATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAACAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482