Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DLM_RS01260 Genome accession   NZ_AP018823
Coordinates   247428..248849 (-) Length   473 a.a.
NCBI ID   WP_089084019.1    Uniprot ID   A0A3G9GCW3
Organism   Aquitalea magnusonii strain H3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 242428..253849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLM_RS01230 (DLM_0249) - 242519..243982 (-) 1464 WP_089084013.1 NAD(P)H-hydrate dehydratase -
  DLM_RS01235 (DLM_0250) - 243986..244606 (-) 621 WP_089084014.1 flavin prenyltransferase UbiX -
  DLM_RS01240 (DLM_0251) rapZ 244674..245516 (-) 843 WP_089084015.1 RNase adapter RapZ -
  DLM_RS01245 (DLM_0252) hprK 245497..246453 (-) 957 WP_089084016.1 HPr(Ser) kinase/phosphatase -
  DLM_RS01250 (DLM_0253) ptsN 246446..246910 (-) 465 WP_089084017.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DLM_RS01255 (DLM_0254) hpf 247032..247355 (-) 324 WP_089084018.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DLM_RS01260 (DLM_0255) rpoN/rpoN1 247428..248849 (-) 1422 WP_089084019.1 RNA polymerase factor sigma-54 Machinery gene
  DLM_RS01265 (DLM_0256) lptB 248853..249584 (-) 732 WP_089084020.1 LPS export ABC transporter ATP-binding protein -
  DLM_RS01270 (DLM_0257) lptA 249581..250135 (-) 555 WP_089084021.1 lipopolysaccharide transport periplasmic protein LptA -
  DLM_RS01275 (DLM_0258) lptC 250125..250685 (-) 561 WP_089084022.1 LPS export ABC transporter periplasmic protein LptC -
  DLM_RS01280 (DLM_0259) - 250682..251206 (-) 525 WP_089084023.1 KdsC family phosphatase -
  DLM_RS01285 (DLM_0260) - 251225..252205 (-) 981 WP_089084024.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 473 a.a.        Molecular weight: 52437.26 Da        Isoelectric Point: 4.7548

>NTDB_id=71533 DLM_RS01260 WP_089084019.1 247428..248849(-) (rpoN/rpoN1) [Aquitalea magnusonii strain H3]
MKQSLQLRVSQQLTLTPQLQQSIKLLQLSTLDLQTEVERFLLDNPMLERGDDLPATDSPSSETASSGEESTTPASDSSDG
DSHSESSGELTDWGSGSRRNDGDDEFDPMLNVPCPVSLREHLLAQLGELTLPTRDQAIVQLLIEELDDNGFLSVDLEELA
SNLPLELELESDELMVGLRLLQQFDPAGIAARSLAESLSLQLDRLPRQEPGHALARKIVAEHLTLLGNRDYTRLKRILGV
DDDAIRQAQQLIARLTPHPAAGFGGEDVHYVIPDVTVRKRRGHWVAELNNGAVPRLRVNQLYAQMLSENRSGAGELTGRL
QEAKWLVKNIQQRFDTILKVSEAIVERQQAFFEHGEVAMRPLILRDIADELGLHESTVSRVTTQKYLLCPRGLFELKYFF
GSALETDSGGECSATAIKAHIRRMIDGENPAKPLSDSAIADELGKQGIQVARRTVAKYREAMQIPPVNLRKAL

Nucleotide


Download         Length: 1422 bp        

>NTDB_id=71533 DLM_RS01260 WP_089084019.1 247428..248849(-) (rpoN/rpoN1) [Aquitalea magnusonii strain H3]
ATGAAACAGTCACTACAGCTCCGGGTTTCACAGCAACTCACCCTCACTCCGCAACTGCAACAGTCGATCAAGCTGCTGCA
GTTGTCCACGCTCGACCTGCAAACCGAGGTGGAGCGCTTCTTGCTGGACAATCCCATGCTGGAGCGCGGCGACGACCTGC
CCGCAACAGACAGCCCCAGCAGCGAAACGGCCAGCAGCGGCGAAGAATCCACCACCCCGGCCTCCGACAGCAGTGATGGC
GACAGCCACAGCGAGAGCAGCGGCGAACTGACCGACTGGGGTAGCGGCAGTCGCCGTAATGATGGCGACGATGAATTCGA
CCCCATGCTCAACGTCCCCTGCCCGGTCAGCCTGCGCGAACACCTGCTGGCGCAATTGGGAGAACTTACCCTCCCCACCC
GCGATCAGGCCATCGTGCAGCTACTGATCGAAGAGCTGGACGACAACGGCTTTCTGAGCGTGGATCTGGAAGAGCTGGCC
AGCAATCTGCCGCTGGAACTGGAACTGGAAAGCGACGAACTGATGGTCGGCTTGCGCCTGCTACAGCAATTCGACCCCGC
CGGCATCGCTGCGCGCAGCCTTGCCGAATCGCTCAGCCTGCAACTGGACCGCCTGCCCCGGCAAGAGCCCGGCCATGCGC
TGGCACGCAAGATTGTGGCCGAACACCTCACCTTGCTTGGCAACCGCGATTACACCCGCCTCAAGCGCATCCTGGGGGTG
GATGACGACGCCATCCGCCAGGCCCAGCAACTGATTGCCAGACTCACCCCGCACCCTGCAGCCGGCTTTGGCGGCGAAGA
CGTACACTATGTCATTCCCGATGTCACCGTGCGCAAACGCCGTGGCCACTGGGTGGCAGAACTGAACAACGGGGCAGTGC
CACGCCTGCGCGTCAATCAGCTATACGCACAGATGCTGTCGGAAAACCGCAGCGGCGCAGGAGAACTCACCGGTCGGCTG
CAAGAAGCAAAATGGCTGGTCAAGAACATCCAGCAACGATTTGACACCATTCTGAAAGTGTCAGAAGCTATAGTCGAAAG
ACAGCAAGCGTTCTTTGAACACGGTGAAGTGGCCATGCGCCCGCTCATCCTGCGCGACATCGCAGACGAGCTGGGACTGC
ATGAATCCACGGTCTCACGCGTCACCACCCAGAAATACCTGCTCTGCCCGCGCGGCCTGTTTGAGCTGAAATACTTTTTT
GGCAGCGCCCTGGAAACAGACAGCGGTGGCGAATGCTCGGCCACCGCCATCAAGGCCCACATCCGCCGCATGATTGATGG
CGAGAACCCGGCCAAGCCACTTTCCGACAGCGCCATCGCCGATGAACTGGGCAAACAAGGCATACAGGTTGCAAGACGCA
CCGTTGCGAAGTATCGTGAAGCTATGCAGATACCGCCGGTCAATCTGCGCAAGGCACTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-302)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-471)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G9GCW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.789

100

0.56

Multiple sequence alignment