Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   LOY65_RS01925 Genome accession   NZ_CP102175
Coordinates   430558..431622 (+) Length   354 a.a.
NCBI ID   WP_024779909.1    Uniprot ID   A0A8B6US63
Organism   Pseudomonas corrugata strain B21-061     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425558..436622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY65_RS01915 (LOY65_01915) - 426383..427651 (+) 1269 WP_024779911.1 malic enzyme-like NAD(P)-binding protein -
  LOY65_RS01920 (LOY65_01920) - 427907..430351 (-) 2445 WP_392396949.1 penicillin-binding protein 1A -
  LOY65_RS01925 (LOY65_01925) comM 430558..431622 (+) 1065 WP_024779909.1 pilus assembly protein PilM Machinery gene
  LOY65_RS01930 (LOY65_01930) - 431622..432188 (+) 567 WP_024779908.1 PilN domain-containing protein -
  LOY65_RS01935 (LOY65_01935) pilO 432185..432808 (+) 624 WP_024779907.1 type 4a pilus biogenesis protein PilO -
  LOY65_RS01940 (LOY65_01940) - 432805..433332 (+) 528 WP_024779906.1 pilus assembly protein PilP -
  LOY65_RS01945 (LOY65_01945) pilQ 433346..435418 (+) 2073 WP_024779905.1 type IV pilus secretin PilQ Machinery gene
  LOY65_RS01950 (LOY65_01950) aroK 435423..435941 (+) 519 WP_024779904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38164.69 Da        Isoelectric Point: 4.9112

>NTDB_id=715202 LOY65_RS01925 WP_024779909.1 430558..431622(+) (comM) [Pseudomonas corrugata strain B21-061]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVIEKNIAELEGVGQALSRVMVKARTSSRSVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVLGASPRNSERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FSNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=715202 LOY65_RS01925 WP_024779909.1 430558..431622(+) (comM) [Pseudomonas corrugata strain B21-061]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGCATCGACATCAGCTCCACATCGGTGAAGCTGCTCGAGTT
GAGCCGCCAGGGTGACCGTTACCGTGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGATCGAGAAGAACA
TCGCCGAGCTCGAGGGGGTCGGGCAGGCGTTATCCCGGGTTATGGTCAAGGCCAGGACGTCTTCGCGCAGCGTGGCGGTG
GCCGTGGCGGGTTCGGCGGTGATCACCAAGACCATCGAGATGGATGCCGGGCTTTCCGACGACGAGATGGAGAACCAGCT
CAAGATCGAGGCGGACCAGTACATTCCTTATCCACTGGATGAAGTCGCCATCGATTTCGAGGTTCTGGGGGCGTCGCCGC
GCAACAGCGAGCGGGTGGAGGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAGGTTCGCGAGGCTGCGCTGGCGCTG
GCGGGGCTGACGGCCCGGGTAGTCGACGTGGAAGCCTACGCGTTGGAGCGCGCCTTCGGCCTGCTTGCCACGCAGCTGGC
CGCGTCCCAGGAGCGTTTGACCGTTGCGGTGATCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGAC
GCATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTCACCGAGGAAATCCAGCGGCGCTACGGCCTGACCCTC
GAACAGGCCGGCCTGGCAAAGAAGCAGGGAGGCTTGCCGGACGATTATCTCAGCGAGGTGCTGCAGCCCTTTCGCGAGGC
ATTGGTGCAGCAGGTTTCGCGGTCCCTGCAGTTTTTCTTCGCTTCGGGCCAGTACAGCGCGGTGGACCATATCCTGCTGG
CCGGTGGTACGGCGTCAGTCGCCGGGCTTGACCGGCTGATCGAGCAACGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTCTCCAACATGGCCCTGGGCAGCAAGGTCAACGCCGGTGCCCTGGCCAGCGATGCGCCGGCGTTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B6US63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486