Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PP477_RS25680 Genome accession   NZ_CP116934
Coordinates   5470688..5472181 (+) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain CUVET20-956     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5465688..5477181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PP477_RS25650 (PP477_25635) - 5465901..5466710 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  PP477_RS25655 (PP477_25640) kdsD 5467123..5468103 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  PP477_RS25660 (PP477_25645) - 5468103..5468642 (+) 540 WP_003094350.1 KdsC family phosphatase -
  PP477_RS25665 (PP477_25650) lptC 5468651..5469223 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  PP477_RS25670 (PP477_25655) lptA 5469210..5469737 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  PP477_RS25675 (PP477_25660) lptB 5469737..5470462 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  PP477_RS25680 (PP477_25665) rpoN/rpoN1 5470688..5472181 (+) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  PP477_RS25685 (PP477_25670) hpf 5472259..5472567 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PP477_RS25690 (PP477_25675) ptsN 5472581..5473045 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PP477_RS25695 (PP477_25680) rapZ 5473047..5473907 (+) 861 WP_003094361.1 RNase adapter RapZ -
  PP477_RS25700 (PP477_25685) - 5473937..5474209 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  PP477_RS25705 (PP477_25690) - 5474306..5475238 (-) 933 WP_019485801.1 ZIP family metal transporter -
  PP477_RS25710 (PP477_25695) - 5475257..5475868 (-) 612 WP_003094367.1 superoxide dismutase -
  PP477_RS25715 (PP477_25700) - 5475881..5476330 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=714740 PP477_RS25680 WP_003094357.1 5470688..5472181(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CUVET20-956]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=714740 PP477_RS25680 WP_003094357.1 5470688..5472181(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CUVET20-956]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGGATCGGCGCTCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477