Detailed information NTG0714437

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NP224_RS23945
• Genome accession: NZ_CP102103
• Coordinates: 5038030..5038827 (+)
• Length: 265 a.a.
• NCBI ID: WP_014227970.1
• Uniprot ID: A0A0H3H8P5
• Organism: Klebsiella michiganensis strain BSI-KPN166
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5033030..5043827

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP224_RS23910 (NP224_23910)	gspH	5033171..5033683 (+)	513	WP_064377768.1	type II secretion system minor pseudopilin GspH	-
NP224_RS23915 (NP224_23915)	gspI	5033680..5034045 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
NP224_RS23920 (NP224_23920)	gspJ	5034042..5034638 (+)	597	WP_047724421.1	type II secretion system minor pseudopilin GspJ	-
NP224_RS23925 (NP224_23925)	gspK	5034635..5035615 (+)	981	WP_064358585.1	type II secretion system minor pseudopilin GspK	-
NP224_RS23930 (NP224_23930)	gspL	5035608..5036801 (+)	1194	WP_064358586.1	type II secretion system protein GspL	-
NP224_RS23935 (NP224_23935)	gspM	5036794..5037279 (+)	486	WP_032694397.1	type II secretion system protein GspM	-
NP224_RS23940 (NP224_23940)	-	5037269..5038027 (+)	759	WP_049083160.1	type II secretion system protein N	-
NP224_RS23945 (NP224_23945)	pilD	5038030..5038827 (+)	798	WP_014227970.1	A24 family peptidase	Machinery gene
NP224_RS23950 (NP224_23950)	hrpB	5039008..5041437 (-)	2430	WP_064358587.1	ATP-dependent helicase HrpB	-
NP224_RS23955 (NP224_23955)	thpR	5041510..5042046 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
NP224_RS23960 (NP224_23960)	sfsA	5042046..5042762 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
NP224_RS23965 (NP224_23965)	dksA	5042927..5043382 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29166.77 Da
• Isoelectric Point: 8.3358

>NTDB_id=714437 NP224_RS23945 WP_014227970.1 5038030..5038827(+) (pilD) [Klebsiella michiganensis strain BSI-KPN166]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=714437 NP224_RS23945 WP_014227970.1 5038030..5038827(+) (pilD) [Klebsiella michiganensis strain BSI-KPN166]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCACTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0H3H8P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434