Detailed information NTG0714366

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: NL420_RS05850
• Genome accession: NZ_CP116925
• Coordinates: 1072797..1073789 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain MLI104K2
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1067797..1078789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NL420_RS05830 (NL420_005830)	otnK	1068572..1069834 (+)	1263	WP_000590403.1	3-oxo-tetronate kinase	-
NL420_RS05835 (NL420_005835)	ygbL	1069831..1070469 (+)	639	WP_001278994.1	aldolase	-
NL420_RS05840 (NL420_005840)	ygbM	1070474..1071250 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
NL420_RS05845 (NL420_005845)	ygbN	1071339..1072703 (+)	1365	WP_000104441.1	GntP family transporter	-
NL420_RS05850 (NL420_005850)	rpoS	1072797..1073789 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
NL420_RS05855 (NL420_005855)	nlpD	1073852..1074946 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
NL420_RS05860 (NL420_005860)	pcm	1075131..1075757 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
NL420_RS05865 (NL420_005865)	surE	1075751..1076512 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
NL420_RS05870 (NL420_005870)	truD	1076493..1077542 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
NL420_RS05875 (NL420_005875)	ispF	1077539..1078018 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
NL420_RS05880 (NL420_005880)	ispD	1078018..1078728 (-)	711	WP_072667568.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=714366 NL420_RS05850 WP_000081550.1 1072797..1073789(-) (rpoS) [Escherichia coli strain MLI104K2]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=714366 NL420_RS05850 WP_000081550.1 1072797..1073789(-) (rpoS) [Escherichia coli strain MLI104K2]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715