Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NP435_RS13815 Genome accession   NZ_CP101937
Coordinates   2631580..2631990 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain RUB331     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2626580..2636990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP435_RS13785 (NP435_13785) - 2626667..2626807 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NP435_RS13790 (NP435_13790) yqeF 2627113..2627844 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NP435_RS13795 (NP435_13795) cwlH 2628096..2628848 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NP435_RS13800 (NP435_13800) yqeD 2629035..2629661 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  NP435_RS13805 (NP435_13805) gnd 2629680..2630573 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NP435_RS13810 (NP435_13810) yqeB 2630825..2631547 (+) 723 WP_010886572.1 hypothetical protein -
  NP435_RS13815 (NP435_13815) nucA/comI 2631580..2631990 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NP435_RS13820 (NP435_13820) sigK 2632186..2632914 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NP435_RS13825 (NP435_13825) - 2632914..2633030 (+) 117 WP_003229898.1 hypothetical protein -
  NP435_RS13830 (NP435_13830) - 2632996..2633196 (-) 201 Protein_2679 recombinase family protein -
  NP435_RS13835 (NP435_13835) - 2633139..2633303 (-) 165 WP_010886576.1 hypothetical protein -
  NP435_RS13840 (NP435_13840) psiE 2633848..2634264 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  NP435_RS13845 (NP435_13845) yrkQ 2634310..2635608 (-) 1299 WP_003246194.1 two-component system sensor histidine kinase YrkQ -
  NP435_RS13850 (NP435_13850) yrkP 2635595..2636290 (-) 696 WP_003229895.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=713335 NP435_RS13815 WP_009967785.1 2631580..2631990(-) (nucA/comI) [Bacillus subtilis strain RUB331]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=713335 NP435_RS13815 WP_009967785.1 2631580..2631990(-) (nucA/comI) [Bacillus subtilis strain RUB331]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529