Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NP436_RS12730 Genome accession   NZ_CP101932
Coordinates   2365245..2366282 (-) Length   345 a.a.
NCBI ID   WP_031600685.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. natto strain BGSC 27E1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2360245..2371282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP436_RS12680 (NP436_12680) tasA 2360343..2361128 (-) 786 WP_014480250.1 biofilm matrix protein TasA -
  NP436_RS12685 (NP436_12685) sipW 2361192..2361764 (-) 573 WP_003230181.1 signal peptidase I SipW -
  NP436_RS12690 (NP436_12690) tapA 2361748..2362509 (-) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  NP436_RS12695 (NP436_12695) yqzG 2362781..2363107 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NP436_RS12700 (NP436_12700) spoIITA 2363149..2363328 (-) 180 WP_014480252.1 YqzE family protein -
  NP436_RS12705 (NP436_12705) comGG 2363399..2363773 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  NP436_RS12710 (NP436_12710) comGF 2363774..2364157 (-) 384 WP_014480254.1 ComG operon protein ComGF Machinery gene
  NP436_RS12715 (NP436_12715) comGE 2364183..2364530 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  NP436_RS12720 (NP436_12720) comGD 2364514..2364945 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  NP436_RS12725 (NP436_12725) comGC 2364935..2365231 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  NP436_RS12730 (NP436_12730) comGB 2365245..2366282 (-) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  NP436_RS12735 (NP436_12735) comGA 2366269..2367339 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NP436_RS12740 (NP436_12740) - 2367551..2367748 (-) 198 WP_014480259.1 CBS domain-containing protein -
  NP436_RS12745 (NP436_12745) corA 2367750..2368703 (-) 954 WP_014480260.1 magnesium transporter CorA -
  NP436_RS12750 (NP436_12750) yqhB 2368845..2370173 (+) 1329 WP_014480261.1 hemolysin family protein -
  NP436_RS12755 (NP436_12755) rsbRD 2370226..2371062 (-) 837 WP_014480262.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39592.99 Da        Isoelectric Point: 9.6259

>NTDB_id=713167 NP436_RS12730 WP_031600685.1 2365245..2366282(-) (comGB) [Bacillus subtilis subsp. natto strain BGSC 27E1]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTGSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKSVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=713167 NP436_RS12730 WP_031600685.1 2365245..2366282(-) (comGB) [Bacillus subtilis subsp. natto strain BGSC 27E1]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGGTTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGTGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.071

93.623

0.928