Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   PNF29_RS14215 Genome accession   NZ_CP116773
Coordinates   2681447..2682796 (+) Length   449 a.a.
NCBI ID   WP_041333853.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM125727     
Function   degrading CSP; selective degradation of ComEA and ComEC (predicted from homology)   
Competence regulation

Genomic Context

Location: 2676447..2687796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS14190 (PNF29_14190) dppD 2676584..2677591 (-) 1008 WP_015252254.1 dipeptide ABC transporter ATP-binding subunit DppD -
  PNF29_RS14195 (PNF29_14195) dppC 2677596..2678558 (-) 963 WP_014476568.1 dipeptide ABC transporter permease DppC -
  PNF29_RS14200 (PNF29_14200) dppB 2678564..2679490 (-) 927 WP_003245446.1 dipeptide ABC transporter permease DppB -
  PNF29_RS14205 (PNF29_14205) dppA 2679507..2680331 (-) 825 WP_014479576.1 D-aminopeptidase DppA -
  PNF29_RS14210 (PNF29_14210) proG 2680460..2681278 (-) 819 WP_041333850.1 pyrroline-5-carboxylate reductase ProG -
  PNF29_RS14215 (PNF29_14215) htrA 2681447..2682796 (+) 1350 WP_041333853.1 serine protease HtrA Regulator
  PNF29_RS14220 (PNF29_14220) - 2682954..2683130 (+) 177 WP_003245413.1 hypothetical protein -
  PNF29_RS14225 (PNF29_14225) gtcC 2683314..2684285 (-) 972 WP_041333856.1 glycosyltransferase family 2 protein -
  PNF29_RS14230 (PNF29_14230) ykcB 2684297..2686447 (-) 2151 WP_272515588.1 glycosyltransferase family 39 protein -
  PNF29_RS14235 (PNF29_14235) - 2686455..2686600 (-) 146 Protein_2812 hypothetical protein -
  PNF29_RS14240 (PNF29_14240) mhqA 2686665..2687615 (-) 951 WP_014476561.1 ring-cleaving dioxygenase -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 47744.87 Da        Isoelectric Point: 4.5990

>NTDB_id=713049 PNF29_RS14215 WP_041333853.1 2681447..2682796(+) (htrA) [Bacillus subtilis strain SRCM125727]
MDNYRDENRTKGNENEVFLTKENDQSTSYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSLALGIYTFTPLGDHDSQDTAK
QSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISDMVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSG
SGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFA
IPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLK
GKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEKTS

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=713049 PNF29_RS14215 WP_041333853.1 2681447..2682796(+) (htrA) [Bacillus subtilis strain SRCM125727]
ATGGATAACTATCGTGATGAAAACAGAACGAAAGGTAATGAGAATGAGGTCTTTTTAACGAAAGAGAACGATCAGAGCAC
CTCCTACTCGGCCCGCAATGTCATTCATGATCAGGAGAAGAAAAAACGAGGATTCGGATGGTTCAGACCGTTGCTTGGCG
GAGTGATCGGCGGCAGTCTTGCCCTTGGCATTTACACGTTTACACCGCTTGGTGACCATGATTCTCAGGACACTGCAAAA
CAATCATCCAGCCAGCAGCAAACGCAATCTGTTACAGCAACAAGCACCTCCTCTGAATCTAAAAAAAGCTCAAGCAGCTC
ATCTGCATTCAAGAGCGAGGACTCTTCTAAAATCTCAGATATGGTAGAAGACCTTTCACCAGCGATTGTCGGTATTACAA
ATCTTCAGGCACAATCAAACAGCTCTTTGTTCGGCTCTAGTTCTTCTGATTCCAGCGAAGATACAGAAAGCGGTTCAGGG
TCAGGTGTCATTTTCAAAAAAGAGAATGGCAAGGCTTATATCATTACAAATAACCACGTCGTAGAAGGGGCATCATCACT
GAAGGTATCTTTATATGACGGCACTGAGGTTACTGCAAAGCTGGTAGGCAGTGACTCGTTAACTGATTTAGCCGTCCTCC
AAATCAGTGATGACCACGTCACAAAAGTGGCAAACTTCGGTGATTCATCTGATCTTAGAACAGGCGAGACCGTTATTGCG
ATTGGGGATCCGCTTGGAAAAGACCTGTCCCGCACAGTAACACAAGGAATTGTAAGCGGCGTGGACAGAACGGTTTCAAT
GTCTACATCAGCCGGCGAAACGAGCATTAACGTCATTCAGACAGACGCAGCAATTAATCCAGGTAACAGCGGCGGTCCTT
TGTTAAATACAGACGGCAAAATTGTCGGCATTAACAGTATGAAAATCAGTGAGGATGATGTTGAGGGTATCGGATTCGCC
ATTCCAAGCAATGACGTAAAACCGATTGCTGAAGAATTGCTGTCTAAAGGACAAATTGAACGTCCATATATCGGTGTCAG
CATGCTTGATCTAGAGCAAGTGCCGCAAAATTACCAAGAAGGCACACTCGGCCTGTTCGGCAGCCAGCTGAATAAAGGCG
TTTACATCCGTGAGGTCGCTTCAGGCTCTCCTGCTGAAAAGGCCGGATTAAAAGCGGAGGATATTATCATCGGCCTAAAA
GGTAAAGAAATTGATACAGGCAGTGAATTGCGCAATATCTTATATAAAGACGCAAAGATCGGTGATACCGTTGAAGTGAA
AATTCTCCGAAACGGCAAAGAAATGACGAAAAAAATTAAACTGGATCAAAAAGAAGAGAAAACTTCGTAA

Domains

Predicted by InterProScan.

PDZ domain (PDZ_2)

(342-441)

Trypsin-like peptidase domain (Trypsin_2)

(160-304)

Peptidase family M50 (Peptidase_M50)

(366-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus pneumoniae Rx1

41.045

89.532

0.367
  htrA Streptococcus pneumoniae D39

41.045

89.532

0.367
  htrA Streptococcus pneumoniae R6

41.045

89.532

0.367
  htrA Streptococcus pneumoniae TIGR4

41.045

89.532

0.367
  htrA Streptococcus mitis NCTC 12261

39.563

91.759

0.363