Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NP433_RS12850 Genome accession   NZ_CP101930
Coordinates   2519236..2519646 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain BGSC 10A5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2514236..2524646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP433_RS12820 (NP433_12820) - 2514323..2514463 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NP433_RS12825 (NP433_12825) yqeF 2514769..2515500 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NP433_RS12830 (NP433_12830) cwlH 2515752..2516504 (-) 753 WP_256682882.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NP433_RS12835 (NP433_12835) yqeD 2516691..2517317 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  NP433_RS12840 (NP433_12840) gnd 2517336..2518229 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NP433_RS12845 (NP433_12845) yqeB 2518481..2519203 (+) 723 WP_010886572.1 hypothetical protein -
  NP433_RS12850 (NP433_12850) nucA/comI 2519236..2519646 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NP433_RS12855 (NP433_12855) sigK 2519842..2520570 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NP433_RS12860 (NP433_12860) - 2520570..2520686 (+) 117 WP_124058645.1 hypothetical protein -
  NP433_RS12865 (NP433_12865) - 2520652..2520848 (-) 197 Protein_2490 recombinase family protein -
  NP433_RS12870 (NP433_12870) - 2520806..2520984 (-) 179 Protein_2491 hypothetical protein -
  NP433_RS12875 (NP433_12875) arsC 2521370..2521789 (-) 420 WP_124058649.1 arsenate reductase (thioredoxin) -
  NP433_RS12880 (NP433_12880) - 2521811..2523109 (-) 1299 WP_227098808.1 arsenic transporter -
  NP433_RS12885 (NP433_12885) - 2523121..2523468 (-) 348 WP_124058652.1 metalloregulator ArsR/SmtB family transcription factor -
  NP433_RS12890 (NP433_12890) - 2523647..2523967 (+) 321 WP_124058654.1 metalloregulator ArsR/SmtB family transcription factor -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=713017 NP433_RS12850 WP_009967785.1 2519236..2519646(-) (nucA/comI) [Bacillus subtilis strain BGSC 10A5]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=713017 NP433_RS12850 WP_009967785.1 2519236..2519646(-) (nucA/comI) [Bacillus subtilis strain BGSC 10A5]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529