Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   PNF29_RS04020 Genome accession   NZ_CP116773
Coordinates   781533..783857 (+) Length   774 a.a.
NCBI ID   WP_272515974.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM125727     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 776533..788857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS04000 (PNF29_04000) cadA 777227..779335 (+) 2109 WP_272515972.1 heavy metal translocating P-type ATPase -
  PNF29_RS04005 (PNF29_04005) bdbD 779455..780123 (+) 669 WP_003228414.1 disulfide bond formation protein BdbD -
  PNF29_RS04010 (PNF29_04010) bdbC 780128..780544 (+) 417 WP_272515973.1 disulfide oxidoreductase -
  PNF29_RS04015 (PNF29_04015) yvgT 780731..781339 (+) 609 WP_003219999.1 trimeric intracellular cation channel family protein -
  PNF29_RS04020 (PNF29_04020) helD 781533..783857 (+) 2325 WP_272515974.1 DNA helicase IV Machinery gene
  PNF29_RS04025 (PNF29_04025) cysJ 784028..785845 (+) 1818 WP_103330121.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  PNF29_RS04030 (PNF29_04030) cysI 785871..787586 (+) 1716 WP_017696388.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89887.47 Da        Isoelectric Point: 6.3819

>NTDB_id=712977 PNF29_RS04020 WP_272515974.1 781533..783857(+) (helD) [Bacillus subtilis strain SRCM125727]
MNQQDKEWKEEQSRIDEVLQELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREQ
NHRRMDQQLKRIHQLKKSPYFGRIDFIENGEEQAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGTLKAMFNSDMTIGDEMLQEVLSQHSDTQMKNIVSTIQKEQNQIIRNEKSKILIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESAFDQLEYCLTE
TKEGDFPTRLAGITWKAGLSFQQFINEYVTRLSSEGMIFKNIIFRGQKLITKEHIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
PQMYLQLFSGWGGKFQHEKVAAIGELTRLAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTVQGDQRMDASFEDEPAEYVRLKRTYRSTRQIVEFTKAMLQDGEDIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGHETIAVICKTAQQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGAASPFVTAVPPHLYQIAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=712977 PNF29_RS04020 WP_272515974.1 781533..783857(+) (helD) [Bacillus subtilis strain SRCM125727]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGCAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCAAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAG
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCAATTAAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGAGCAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTTTCTGA
TTTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCCCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCACTCTCAAGGCGATGTTTAATTCCGATAT
GACGATTGGCGATGAAATGCTGCAAGAGGTATTAAGCCAGCACTCCGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGATCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGCGTCATTGATGCCGGACAAATCGTTTTGTTTTC
TCCCAACTTCTTATTTAACAGCTATGTATCCTCTGTTTTGCCTGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCGCCTTTGATCAGCTCGAATATTGCCTGACTGAA
ACAAAGGAAGGCGATTTTCCAACAAGGCTTGCGGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTAATGA
ATACGTCACACGGCTTTCCTCTGAAGGGATGATTTTTAAAAATATTATATTCCGCGGACAAAAGCTGATCACAAAGGAAC
ACATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAACCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTCGTTCATGAAGCTGAGCTGCTCGACAAAGAGGA
CTACTTGGACGTGTATAAGAAACTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAACAAGCGGTCAGGCTCCTTGCATTTCTTGATGTT
CCACAGATGTATTTGCAATTGTTTTCTGGCTGGGGAGGAAAATTTCAGCATGAGAAGGTGGCCGCGATAGGTGAGCTGAC
CCGTTTGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAGATCAAACATCTATTTATTGACGAAGCCCAAGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGCATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCGTACAAGGGGATCA
GCGTATGGATGCGAGCTTTGAGGATGAGCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAATTTACCAAAGCGATGCTTCAAGATGGAGAGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGACGGCTCAAGAAAAAAGGGCACGAAACAAT
AGCCGTCATCTGCAAGACTGCGCAACAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTCCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCGGTATTTTATACAGGTGCAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCACTTATATCAGATTGCTG
AATGA

Domains

Predicted by InterProScan.

AAA domain (AAA_19)

(217-580)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

97.674

100

0.977