Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   NPS25_RS18415 Genome accession   NZ_CP101904
Coordinates   3752752..3753897 (+) Length   381 a.a.
NCBI ID   WP_015418439.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ba-0321     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3747752..3758897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPS25_RS18395 (NPS25_18395) - 3749318..3749746 (-) 429 WP_060673887.1 DUF1934 domain-containing protein -
  NPS25_RS18400 (NPS25_18400) - 3749900..3750412 (+) 513 Protein_3567 bacteriocin biosynthesis protein AlbG -
  NPS25_RS18405 (NPS25_18405) - 3750419..3751126 (-) 708 WP_061890880.1 YncE family protein -
  NPS25_RS18410 (NPS25_18410) - 3751166..3752521 (-) 1356 WP_015418438.1 YncE family protein -
  NPS25_RS18415 (NPS25_18415) rapF 3752752..3753897 (+) 1146 WP_015418439.1 tetratricopeptide repeat protein Regulator
  NPS25_RS18420 (NPS25_18420) phrF 3753881..3754000 (+) 120 WP_015240778.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  NPS25_RS18425 (NPS25_18425) - 3754198..3754401 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  NPS25_RS18430 (NPS25_18430) - 3754415..3754618 (+) 204 WP_007407641.1 hypothetical protein -
  NPS25_RS18435 (NPS25_18435) - 3754923..3755204 (+) 282 WP_045209208.1 hypothetical protein -
  NPS25_RS18440 (NPS25_18440) - 3755248..3755760 (+) 513 WP_031378560.1 stage II sporulation protein M -
  NPS25_RS18445 (NPS25_18445) - 3756351..3756569 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  NPS25_RS18450 (NPS25_18450) speB 3756737..3757609 (-) 873 WP_007407646.1 agmatinase -
  NPS25_RS18455 (NPS25_18455) speE 3757668..3758498 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45569.65 Da        Isoelectric Point: 4.5769

>NTDB_id=712614 NPS25_RS18415 WP_015418439.1 3752752..3753897(+) (rapF) [Bacillus velezensis strain Ba-0321]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=712614 NPS25_RS18415 WP_015418439.1 3752752..3753897(+) (rapF) [Bacillus velezensis strain Ba-0321]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCATAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTATTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564