Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   NPS25_RS12295 Genome accession   NZ_CP101904
Coordinates   2549834..2550634 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain Ba-0321     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2544834..2555634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPS25_RS12265 (NPS25_12265) - 2545374..2546006 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  NPS25_RS12270 (NPS25_12270) - 2546303..2546440 (+) 138 WP_014470646.1 hypothetical protein -
  NPS25_RS12275 (NPS25_12275) - 2546534..2547652 (+) 1119 WP_032863662.1 NADH-dependent flavin oxidoreductase -
  NPS25_RS12280 (NPS25_12280) - 2547775..2547990 (+) 216 WP_007612497.1 spore germination protein -
  NPS25_RS12285 (NPS25_12285) - 2548116..2548328 (+) 213 WP_003153181.1 spore germination protein -
  NPS25_RS12290 (NPS25_12290) alr 2548504..2549685 (+) 1182 WP_012117960.1 alanine racemase -
  NPS25_RS12295 (NPS25_12295) spo0A 2549834..2550634 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  NPS25_RS12300 (NPS25_12300) spoIVB 2550916..2552196 (-) 1281 WP_061890717.1 SpoIVB peptidase -
  NPS25_RS12305 (NPS25_12305) recN 2552360..2554090 (-) 1731 WP_039254521.1 DNA repair protein RecN Machinery gene
  NPS25_RS12310 (NPS25_12310) ahrC 2554122..2554571 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  NPS25_RS12315 (NPS25_12315) - 2554668..2555513 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=712580 NPS25_RS12295 WP_003153177.1 2549834..2550634(-) (spo0A) [Bacillus velezensis strain Ba-0321]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=712580 NPS25_RS12295 WP_003153177.1 2549834..2550634(-) (spo0A) [Bacillus velezensis strain Ba-0321]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATTCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACTGCAAGCCGTGTAGAACGGG
CCATCCGGCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATCGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97