Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NPS25_RS01940 Genome accession   NZ_CP101904
Coordinates   391410..391847 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ba-0321     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 386410..396847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPS25_RS01915 (NPS25_01915) - 387430..388566 (+) 1137 WP_025284207.1 zinc-dependent alcohol dehydrogenase -
  NPS25_RS01920 (NPS25_01920) - 388581..389015 (+) 435 WP_007409358.1 RDD family protein -
  NPS25_RS01925 (NPS25_01925) - 389132..389410 (+) 279 WP_263612330.1 YckD family protein -
  NPS25_RS01930 (NPS25_01930) - 389514..390950 (+) 1437 WP_256683712.1 glycoside hydrolase family 1 protein -
  NPS25_RS01935 (NPS25_01935) nin/comJ 390991..391389 (-) 399 WP_256683713.1 competence protein ComJ Regulator
  NPS25_RS01940 (NPS25_01940) nucA/comI 391410..391847 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NPS25_RS01945 (NPS25_01945) hxlB 392178..392735 (-) 558 WP_256683714.1 6-phospho-3-hexuloisomerase -
  NPS25_RS01950 (NPS25_01950) hxlA 392732..393367 (-) 636 WP_015416785.1 3-hexulose-6-phosphate synthase -
  NPS25_RS01955 (NPS25_01955) - 393599..393961 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=712549 NPS25_RS01940 WP_003156588.1 391410..391847(-) (nucA/comI) [Bacillus velezensis strain Ba-0321]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=712549 NPS25_RS01940 WP_003156588.1 391410..391847(-) (nucA/comI) [Bacillus velezensis strain Ba-0321]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGTTACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641