Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NPA43_RS11425 Genome accession   NZ_CP101833
Coordinates   2249265..2250035 (-) Length   256 a.a.
NCBI ID   WP_060697377.1    Uniprot ID   A0A2G8IVY9
Organism   Bacillus pumilus strain NDY-10     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2244265..2255035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS11405 (NPA43_11405) - 2244357..2245178 (-) 822 WP_099726612.1 pyruvate, water dikinase regulatory protein -
  NPA43_RS11410 (NPA43_11410) - 2245204..2245842 (-) 639 WP_099726784.1 helix-turn-helix transcriptional regulator -
  NPA43_RS11415 (NPA43_11415) glyS 2246009..2248054 (-) 2046 WP_230030403.1 glycine--tRNA ligase subunit beta -
  NPA43_RS11420 (NPA43_11420) glyQ 2248047..2248934 (-) 888 WP_003217487.1 glycine--tRNA ligase subunit alpha -
  NPA43_RS11425 (NPA43_11425) recO 2249265..2250035 (-) 771 WP_060697377.1 DNA repair protein RecO Machinery gene
  NPA43_RS11430 (NPA43_11430) - 2250071..2250214 (-) 144 WP_003217288.1 YqzL family protein -
  NPA43_RS11435 (NPA43_11435) era 2250362..2251267 (-) 906 WP_099726610.1 GTPase Era -
  NPA43_RS11440 (NPA43_11440) - 2251260..2251658 (-) 399 WP_008356263.1 cytidine deaminase -
  NPA43_RS11445 (NPA43_11445) - 2251772..2252158 (-) 387 WP_099726609.1 diacylglycerol kinase family protein -
  NPA43_RS11450 (NPA43_11450) ybeY 2252136..2252615 (-) 480 WP_099726608.1 rRNA maturation RNase YbeY -
  NPA43_RS11455 (NPA43_11455) - 2252616..2254727 (-) 2112 WP_370461107.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29258.91 Da        Isoelectric Point: 8.1204

>NTDB_id=712238 NPA43_RS11425 WP_060697377.1 2249265..2250035(-) (recO) [Bacillus pumilus strain NDY-10]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=712238 NPA43_RS11425 WP_060697377.1 2249265..2250035(-) (recO) [Bacillus pumilus strain NDY-10]
ATGCTCATTAAAAGTGAAGGAATCGTTCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAGCATGGGAAAATTGGTGTGATGGCAAGAGGTGCTAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTTATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGTGAAATGATCGAAAGCATGCGGGCC
ATCCGGGAAGATTTATTTCTGACTGCATATGCCGCATATATGACAGAATTACTCGATAAAGGAACGGAGGAAAAGAAGCC
AAACCCTTATTTGTTTGAGCTCCTTCTTCAATCTTTACGTCATCTCAATGAAGGCACAGATGCTGATATTATTTTATTTA
TTGTAGAGGTTAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTGGATCAATGTGTACACTGCGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATTAGAGATAACGGATTTATTTGCCAAAATTGCTTTTCTAAAGATCCATATAAACTGCCCTTATC
CCCAGCAGCTGCAAGACTGCTTCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAAGTTGACGTGAAACCGCAGA
CTAAACAAGAAATTCGTCAAGTGCTGGATCACTACTATGACGAGTATTCAGGCCTTTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G8IVY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754