Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NPA43_RS08620 Genome accession   NZ_CP101833
Coordinates   1730871..1731251 (+) Length   126 a.a.
NCBI ID   WP_230030533.1    Uniprot ID   -
Organism   Bacillus pumilus strain NDY-10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1725871..1736251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS08610 (NPA43_08610) - 1726351..1729494 (+) 3144 WP_256499609.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  NPA43_RS08615 (NPA43_08615) - 1729689..1730597 (+) 909 WP_099725722.1 serine protease -
  NPA43_RS08620 (NPA43_08620) nucA/comI 1730871..1731251 (+) 381 WP_230030533.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NPA43_RS08625 (NPA43_08625) - 1731389..1732237 (+) 849 WP_256499610.1 STAS domain-containing protein -
  NPA43_RS08630 (NPA43_08630) - 1732270..1732812 (-) 543 WP_099725719.1 IseA DL-endopeptidase inhibitor family protein -
  NPA43_RS08635 (NPA43_08635) - 1733107..1733556 (+) 450 WP_099725718.1 hypothetical protein -
  NPA43_RS08640 (NPA43_08640) lexA 1733604..1734224 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  NPA43_RS08645 (NPA43_08645) yneA 1734384..1734695 (+) 312 WP_034660770.1 cell division suppressor protein YneA -
  NPA43_RS08650 (NPA43_08650) - 1734713..1735360 (+) 648 WP_099725717.1 recombinase family protein -
  NPA43_RS08655 (NPA43_08655) - 1735430..1735660 (+) 231 WP_099725716.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13799.48 Da        Isoelectric Point: 7.9018

>NTDB_id=712231 NPA43_RS08620 WP_230030533.1 1730871..1731251(+) (nucA/comI) [Bacillus pumilus strain NDY-10]
MGVLLSGFGENQAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADVRRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=712231 NPA43_RS08620 WP_230030533.1 1730871..1731251(+) (nucA/comI) [Bacillus pumilus strain NDY-10]
ATGGGTGTGTTATTAAGTGGATTTGGAGAGAATCAGGCGGCAAAAGGGGCAGATCGTTATGATCATGTCATTCAATTTCC
AAAGGAACGGTATCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAACGGGGCAGATGTCCGCAGGCAAGAATCGTTAAAAGGAATTCCCACAAAGCCTGGCTTTGACCGGGATGAATGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAGGGTGCAAGTGTTCAATATGTCAGTCCATCAGATAATAGAGGAGCAGGCTCATG
GGTCGGACACCAAATAAGCGGCTATCCTGATGGAAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.824

94.444

0.556