Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NPA43_RS01925 Genome accession   NZ_CP101833
Coordinates   353319..353747 (-) Length   142 a.a.
NCBI ID   WP_099726096.1    Uniprot ID   A0A2G8IXC4
Organism   Bacillus pumilus strain NDY-10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 348319..358747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS01890 (NPA43_01890) - 348472..349149 (-) 678 WP_099726103.1 amino acid ABC transporter permease -
  NPA43_RS01895 (NPA43_01895) - 349162..349977 (-) 816 WP_370461129.1 transporter substrate-binding domain-containing protein -
  NPA43_RS01900 (NPA43_01900) - 350217..350546 (+) 330 WP_099726101.1 YckD family protein -
  NPA43_RS01905 (NPA43_01905) - 350593..351360 (-) 768 WP_256499288.1 MBL fold metallo-hydrolase -
  NPA43_RS01910 (NPA43_01910) - 351477..351893 (+) 417 WP_180275460.1 Lrp/AsnC family transcriptional regulator -
  NPA43_RS01915 (NPA43_01915) - 351921..352727 (+) 807 WP_099726098.1 aminoglycoside N(3)-acetyltransferase -
  NPA43_RS01920 (NPA43_01920) - 352729..353115 (-) 387 WP_099726097.1 competence protein ComJ -
  NPA43_RS01925 (NPA43_01925) nucA/comI 353319..353747 (-) 429 WP_099726096.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NPA43_RS01930 (NPA43_01930) - 354115..356184 (+) 2070 WP_099726094.1 hydantoinase/oxoprolinase family protein -
  NPA43_RS01935 (NPA43_01935) - 356174..358159 (+) 1986 WP_256499289.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15996.99 Da        Isoelectric Point: 6.0964

>NTDB_id=712205 NPA43_RS01925 WP_099726096.1 353319..353747(-) (nucA/comI) [Bacillus pumilus strain NDY-10]
MKLLKTILLLLLIVIGVATGYIQLEQSKQETTNSSYDKTIHFPSDRYPETAKHIEEAIEEGQSSICTIDRKNSDEQRDRS
LHGIPTKRGYDRDEWPMAMCKEGGKGASVKYVRPSDNRGAGSWVSHQLSDDPDGTKIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=712205 NPA43_RS01925 WP_099726096.1 353319..353747(-) (nucA/comI) [Bacillus pumilus strain NDY-10]
ATGAAACTTCTTAAAACCATTCTCTTGCTTTTATTGATTGTCATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAAG
TAAACAAGAGACGACGAATTCATCTTACGACAAAACCATTCACTTTCCATCAGATCGTTATCCAGAAACAGCGAAGCACA
TAGAAGAGGCAATTGAGGAAGGTCAATCCTCCATTTGTACAATTGATCGTAAAAATAGCGATGAGCAGAGAGACCGCTCA
CTTCATGGCATCCCGACAAAGCGCGGATATGACCGGGACGAATGGCCGATGGCGATGTGTAAAGAAGGGGGCAAAGGAGC
TTCTGTTAAATATGTACGTCCTTCAGATAACAGAGGGGCAGGCTCTTGGGTAAGTCATCAATTATCAGATGATCCTGATG
GCACAAAAATTCAATTTATCATTGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G8IXC4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.868

74.648

0.514