Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   NPA43_RS00020 Genome accession   NZ_CP101833
Coordinates   3256..4368 (+) Length   370 a.a.
NCBI ID   WP_099726328.1    Uniprot ID   A0A2G8IWL3
Organism   Bacillus pumilus strain NDY-10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS00005 (NPA43_00005) dnaA 200..1540 (+) 1341 WP_099726331.1 chromosomal replication initiator protein DnaA -
  NPA43_RS00010 (NPA43_00010) dnaN 1737..2873 (+) 1137 WP_249704744.1 DNA polymerase III subunit beta -
  NPA43_RS00015 (NPA43_00015) yaaA 3024..3239 (+) 216 WP_099726329.1 S4 domain-containing protein YaaA -
  NPA43_RS00020 (NPA43_00020) recF 3256..4368 (+) 1113 WP_099726328.1 DNA replication/repair protein RecF Machinery gene
  NPA43_RS00025 (NPA43_00025) remB 4386..4631 (+) 246 WP_099726327.1 extracellular matrix regulator RemB -
  NPA43_RS00030 (NPA43_00030) gyrB 4689..6605 (+) 1917 WP_099726326.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  NPA43_RS00035 (NPA43_00035) gyrA 6836..9340 (+) 2505 WP_256499197.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42555.61 Da        Isoelectric Point: 7.1667

>NTDB_id=712200 NPA43_RS00020 WP_099726328.1 3256..4368(+) (recF) [Bacillus pumilus strain NDY-10]
MYIQSLALTSYRNYEHTELHFDNKVNVMIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDEDYAKIEGRVIKKNGP
LPMQLVISKKGKKGKVNHIEQQKLSHYVGALNTIMFAPEDLSLVKGSPQIRRRFLDMEIGQVSAVYLHDLSLYQKILSQR
NHYLKQLQTRKQTDQAMLEVLTEQLIDAAAKVVKRRLTFTKQLEKWAQPLHFGISRELETLTLQYQTAIEVSEASDLSKI
KNSYEESFQKLRDREIDRGVTLWGPHRDDLLFFVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVEGIDHATLKEAEIFRVASGKVID

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=712200 NPA43_RS00020 WP_099726328.1 3256..4368(+) (recF) [Bacillus pumilus strain NDY-10]
ATGTACATTCAAAGTCTGGCGTTAACCTCATACCGAAACTATGAACACACCGAACTTCATTTCGACAATAAGGTCAATGT
CATGATCGGTGAGAACGCCCAAGGTAAAACGAACTTGATGGAAGCGATCTATGTATTGTCTATGGCAAAGTCTCATCGTA
CGTCAAATGATAAAGAACTTATCCGATGGGACGAAGACTATGCTAAAATAGAAGGTAGAGTCATCAAAAAAAACGGTCCA
CTCCCAATGCAGCTCGTGATCTCAAAAAAAGGAAAAAAGGGAAAGGTCAATCACATTGAACAACAGAAACTTAGTCATTA
TGTTGGTGCTTTAAACACCATCATGTTTGCACCAGAGGATCTGAGCCTTGTGAAAGGCAGCCCGCAAATTCGCAGAAGAT
TCCTCGACATGGAGATTGGACAAGTTTCTGCTGTCTATTTGCATGATTTATCGCTCTATCAAAAAATCCTCTCTCAGCGA
AATCATTACTTGAAACAATTGCAAACAAGAAAGCAAACGGATCAAGCGATGCTGGAGGTTTTAACAGAGCAGTTGATTGA
TGCGGCAGCGAAAGTTGTCAAAAGACGACTTACTTTTACGAAACAGCTTGAAAAATGGGCGCAGCCGTTGCATTTTGGAA
TTTCTAGAGAGCTAGAAACACTCACGCTCCAATACCAGACAGCGATAGAGGTATCAGAAGCGTCAGACTTGTCGAAAATA
AAGAATAGCTATGAAGAATCGTTTCAGAAACTAAGAGACAGAGAAATAGACCGAGGGGTGACACTGTGGGGACCTCACAG
AGATGACCTTCTTTTCTTTGTGAATGGTCGGGATGTTCAGACATATGGCTCTCAAGGGCAGCAAAGAACAACAGCTCTTT
CACTAAAACTTGCAGAAATTGATCTGATCCATGAAGAAATCGGTGAATACCCAATTCTTCTACTCGATGATGTTTTATCT
GAACTTGATGATTATAGACAGTCTCATTTGCTCCATACCATTCAGGGACGTGTACAGACCTTCGTCACGACAACAAGTGT
TGAAGGCATCGATCACGCTACCCTGAAAGAAGCGGAAATTTTCAGAGTAGCCAGTGGAAAAGTAATTGACTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G8IWL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

83.469

99.73

0.832