Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NPC72_RS12045 Genome accession   NZ_CP101831
Coordinates   2499528..2500892 (-) Length   454 a.a.
NCBI ID   WP_003567254.1    Uniprot ID   A0A829GUQ3
Organism   Lacticaseibacillus paracasei strain SMN-LBK     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2494528..2505892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPC72_RS12020 (NPC72_12020) - 2494695..2495615 (-) 921 WP_003574021.1 IS30 family transposase -
  NPC72_RS12025 (NPC72_12025) - 2495725..2496294 (-) 570 Protein_2355 ABC transporter permease -
  NPC72_RS12030 (NPC72_12030) - 2496312..2497199 (-) 888 WP_003567248.1 ABC transporter ATP-binding protein -
  NPC72_RS12035 (NPC72_12035) - 2497383..2498278 (+) 896 Protein_2357 helix-turn-helix domain-containing protein -
  NPC72_RS12040 (NPC72_12040) - 2498391..2499506 (-) 1116 WP_003567252.1 PIN/TRAM domain-containing protein -
  NPC72_RS12045 (NPC72_12045) radA 2499528..2500892 (-) 1365 WP_003567254.1 DNA repair protein RadA Machinery gene
  NPC72_RS12050 (NPC72_12050) - 2500909..2501451 (-) 543 WP_003567256.1 dUTP diphosphatase -
  NPC72_RS12055 (NPC72_12055) - 2501672..2501962 (+) 291 WP_003567258.1 GNAT family N-acetyltransferase -
  NPC72_RS12060 (NPC72_12060) - 2502078..2502455 (-) 378 WP_003567260.1 DUF805 domain-containing protein -
  NPC72_RS12065 (NPC72_12065) - 2502700..2504019 (+) 1320 WP_003588717.1 C1 family peptidase -
  NPC72_RS12070 (NPC72_12070) - 2504342..2505688 (+) 1347 WP_003576225.1 aminopeptidase C -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49126.47 Da        Isoelectric Point: 7.4216

>NTDB_id=712193 NPC72_RS12045 WP_003567254.1 2499528..2500892(-) (radA) [Lacticaseibacillus paracasei strain SMN-LBK]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTAAGSKVKPTRMNDVTITKETRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLANTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=712193 NPC72_RS12045 WP_003567254.1 2499528..2500892(-) (radA) [Lacticaseibacillus paracasei strain SMN-LBK]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGTCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACAGCAGCCGGCAGTAAGG
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGAAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTGCTATTACA
AGTATCAGGGCAGTTGGCTAATACCGGCGGAACCGTTTTGTATGTCTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGTGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTACGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGTGAAGTGACGGCAGAACTGATGCGCATTGCCAAGTCCAAAGGCGTGACCATTTTCATCGTTGGCC
ATGTCACTAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTTGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGGCCAATCTTAGTCGAGATTCAAGCCTTGATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGAGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGGCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAACTGGGTTTCAAACGGATTTTTGTACCACGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A829GUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.212

99.78

0.681
  radA Streptococcus pneumoniae Rx1

68.212

99.78

0.681
  radA Streptococcus pneumoniae D39

68.212

99.78

0.681
  radA Streptococcus pneumoniae R6

68.212

99.78

0.681
  radA Streptococcus pneumoniae TIGR4

68.212

99.78

0.681
  radA Streptococcus mitis SK321

67.991

99.78

0.678
  radA Bacillus subtilis subsp. subtilis str. 168

64.758

100

0.648