Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NPA28_RS13280 Genome accession   NZ_CP101718
Coordinates   2617573..2617998 (-) Length   141 a.a.
NCBI ID   WP_256766241.1    Uniprot ID   -
Organism   Bacillus halotolerans strain S-5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2612573..2622998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA28_RS13250 (NPA28_13250) - 2612813..2612947 (-) 135 WP_010335019.1 hypothetical protein -
  NPA28_RS13255 (NPA28_13255) - 2613025..2613756 (-) 732 WP_044154481.1 SGNH/GDSL hydrolase family protein -
  NPA28_RS13260 (NPA28_13260) - 2614003..2614755 (-) 753 WP_059335450.1 N-acetylmuramoyl-L-alanine amidase -
  NPA28_RS13265 (NPA28_13265) - 2614950..2615576 (+) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  NPA28_RS13270 (NPA28_13270) gnd 2615597..2616490 (-) 894 WP_044154479.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NPA28_RS13275 (NPA28_13275) - 2616811..2617539 (+) 729 WP_044154478.1 hypothetical protein -
  NPA28_RS13280 (NPA28_13280) nucA/comI 2617573..2617998 (-) 426 WP_256766241.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NPA28_RS13285 (NPA28_13285) sigK 2618175..2618903 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NPA28_RS13290 (NPA28_13290) - 2619024..2619197 (-) 174 Protein_2582 recombinase family protein -
  NPA28_RS13295 (NPA28_13295) - 2619155..2619333 (-) 179 Protein_2583 hypothetical protein -
  NPA28_RS13300 (NPA28_13300) - 2619688..2620107 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  NPA28_RS13305 (NPA28_13305) acr3 2620119..2621159 (-) 1041 WP_131228307.1 arsenite efflux transporter Acr3 -
  NPA28_RS13310 (NPA28_13310) - 2621181..2621621 (-) 441 WP_044154474.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  NPA28_RS13315 (NPA28_13315) arsR 2621680..2621997 (-) 318 WP_044154472.1 arsenical resistance operon transcriptional regulator ArsR -
  NPA28_RS13320 (NPA28_13320) - 2622360..2622727 (-) 368 Protein_2588 YqcI/YcgG family protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15526.71 Da        Isoelectric Point: 5.2215

>NTDB_id=711773 NPA28_RS13280 WP_256766241.1 2617573..2617998(-) (nucA/comI) [Bacillus halotolerans strain S-5]
MKRWMAMLLLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGAKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=711773 NPA28_RS13280 WP_256766241.1 2617573..2617998(-) (nucA/comI) [Bacillus halotolerans strain S-5]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCGGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAGTCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCGGATGGCG
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.484

87.943

0.532